Please use this identifier to cite or link to this item: doi:10.22028/D291-35771
Title: Attenuated asthma phenotype in mice with a fetal-like antigen receptor repertoire
Author(s): Stutz, Regine
Meyer, Christopher
Kaiser, Elisabeth
Goedicke-Fritz, Sybelle
Schroeder, Harry W.
Bals, Robert
Haertel, Christoph
Rogosch, Tobias
Kerzel, Sebastian
Zemlin, Michael
Language: English
Title: Scientific Reports
Volume: 11
Issue: 1
Publisher/Platform: Springer Nature
Year of Publication: 2021
Free key words: Immunogenetics
Immunological disorders
Innate immunity
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: We hypothesized that the scarcity of N-nucleotides might contribute to the inability of the neonate to mount a robust allergic immune response. To test this, we used terminal deoxyribunucleotidyl Transferase defcient (TdT−/−) mice, which express “fetal-like” T cell receptor and immunoglobulin repertoires with largely germline-encoded CDR3 regions. Intraperitoneal sensitization was followed by aerosol provocation with either PBS or the allergen OVA in both TdT−/− mice and wild-type mice to develop allergic respiratory infammation. The efects of this procedure were investigated by lung function test, immunological analysis of serum and brochoalveolar lavage. The local TH2 cytokine milieu was signifcantly attenuated in TdT−/− mice. Within this group, the induction of total IgE levels was also signifcantly reduced after sensitization. TdT−/− mice showed a tendency toward reduced eosinophilic infow into the bronchial tubes, which was associated with the elimination of respiratory hyperreactivity. In conclusion, in a murine model of allergic airway infammation, the expression of fetal-like antigen receptors was associated with potent indications of a reduced ability to mount an asthma phenotype. This underlines the importance of somatically-generated antigen-receptor repertoire diversity in type one allergic immune responses and suggests that the fetus may be protected from allergic responses, at least in part, by controlling N addition.
DOI of the first publication: 10.1038/s41598-021-93553-6
Link to this record: urn:nbn:de:bsz:291--ds-357710
ISSN: 2045-2322
Date of registration: 17-Mar-2022
Description of the related object: Supplementary Information
Related object:
Faculty: M - Medizinische Fakultät
Department: M - Innere Medizin
M - Pädiatrie
Professorship: M - Prof. Dr. Robert Bals
M - Prof. Dr. Michael Zemlin
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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