Please use this identifier to cite or link to this item: doi:10.22028/D291-35750
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Title: Light-Sheet Scattering Microscopy to Visualize Long-Term Interactions Between Cells and Extracellular Matrix
Author(s): Zhou, Xiangda
Zhao, Renping
Yanamandra, Archana K.
Hoth, Markus
Qu, Bin
Language: English
Title: Frontiers in immunology
Volume: 13
Startpage: 1
Endpage: 10
Publisher/Platform: Frontiers
Year of Publication: 2022
Publikation type: Journal Article
Abstract: Visualizing interactions between cells and the extracellular matrix (ECM) mesh is important to understand cell behavior and regulatory mechanisms by the extracellular environment. However, long term visualization of three-dimensional (3D) matrix structures remains challenging mainly due to photobleaching or blind spots perpendicular to the imaging plane. Here, we combine label-free light-sheet scattering microcopy (LSSM) and fluorescence microscopy to solve these problems. We verified that LSSM can reliably visualize structures of collagen matrices from different origin including bovine, human and rat tail. The quality and intensity of collagen structure images acquired by LSSM did not decline with time. LSSM offers abundant wavelength choice to visualize matrix structures, maximizing combination possibilities with fluorescently-labelled cells, allowing visualizing of long-term ECM-cell interactions in 3D. Interestingly, we observed ultrathin thread-like structures between cells and matrix using LSSM, which were not observed by normal fluorescence microscopy. Transient local alignment of matrix by cell-applied forces can be observed. In summary, LSSM provides a powerful and robust approach to investigate the complex interplay between cells and ECM.
DOI of the first publication: 10.3389/fimmu.2022.828634
URL of the first publication: https://www.frontiersin.org/articles/10.3389/fimmu.2022.828634/full
Link to this record: hdl:20.500.11880/32601
http://dx.doi.org/10.22028/D291-35750
ISSN: 1664-3224
Date of registration: 15-Mar-2022
Faculty: M - Medizinische Fakultät
Department: M - Biophysik
Professorship: M - Prof. Dr. Markus Hoth
Collections:Die Universitätsbibliographie

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