Please use this identifier to cite or link to this item: doi:10.22028/D291-35474
Title: Bioadhesion on Textured Interfaces in the Human Oral Cavity—An In Situ Study
Author(s): Helbig, Ralf
Hannig, Matthias
Basche, Sabine
Ortgies, Janis
Killge, Sebastian
Hannig, Christian
Sterzenbach, Torsten
Language: English
Title: International Journal of Molecular Sciences
Volume: 23
Issue: 3
Publisher/Platform: MDPI
Year of Publication: 2022
Free key words: textured surfaces
restorative dentistry
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Extensive biofilm formation on materials used in restorative dentistry is a common reason for their failure and the development of oral diseases like peri-implantitis or secondary caries. Therefore, novel materials and strategies that result in reduced biofouling capacities are urgently sought. Previous research suggests that surface structures in the range of bacterial cell sizes seem to be a promising approach to modulate bacterial adhesion and biofilm formation. Here we investigated bioadhesion within the oral cavity on a low surface energy material (perfluorpolyether) with different texture types (line-, hole-, pillar-like), feature sizes in a range from 0.7–4.5 µm and graded distances (0.7–130.5 µm). As a model system, the materials were fixed on splints and exposed to the oral cavity. We analyzed the enzymatic activity of amylase and lysozyme, pellicle formation, and bacterial colonization after 8 h intraoral exposure. In opposite to in vitro experiments, these in situ experiments revealed no clear signs of altered bacterial surface colonization regarding structure dimensions and texture types compared to unstructured substrates or natural enamel. In part, there seemed to be a decreasing trend of adherent cells with increasing periodicities and structure sizes, but this pattern was weak and irregular. Pellicle formation took place on all substrates in an unaltered manner. However, pellicle formation was most pronounced within recessed areas thereby partially masking the three-dimensional character of the surfaces. As the natural pellicle layer is obviously the most dominant prerequisite for bacterial adhesion, colonization in the oral environment cannot be easily controlled by structural means.
DOI of the first publication: 10.3390/ijms23031157
Link to this record: urn:nbn:de:bsz:291--ds-354747
ISSN: 1422-0067
Date of registration: 17-Feb-2022
Description of the related object: Supplementary Materials
Related object:
Faculty: M - Medizinische Fakultät
Department: M - Zahn-, Mund- und Kieferheilkunde
Professorship: M - Prof. Dr. Matthias Hannig
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

Files for this record:
File Description SizeFormat 
ijms-23-01157.pdf4,15 MBAdobe PDFView/Open

This item is licensed under a Creative Commons License Creative Commons