Please use this identifier to cite or link to this item: doi:10.22028/D291-35469
Title: High-Resolution Phosphorescence Lifetime Imaging (PLIM) of Bones
Author(s): Breunig, Hans Georg
König, Karsten
Language: English
Title: Applied Sciences
Volume: 12
Issue: 3
Publisher/Platform: MDPI
Year of Publication: 2022
Free key words: phosphorescence
multiphoton tomography
two-photon imaging
DDC notations: 500 Science
Publikation type: Journal Article
Abstract: For the first time, the time-resolved two-photon excited autophosphorescence of non labeled biological specimens was investigated by phosphoresce lifetime imaging with microscopic spatial resolution. A modified multiphoton tomograph was employed to record both photolumi nescence contributions, autofluorescence and autophosphorescence, simultaneously, induced by two-photon excitation using an 80 MHz near infrared femtosecond-pulse-laser scanning beam, an acousto-optic modulator, and a time-correlated single-photon counting module for lifetime mea surements from the picosecond to the microsecond range. In particular, the two-photon-excited luminescence of thermally altered bones was imaged. A strong dependence of the phosphores cence intensity on exposure temperature, with a maximum emission for an exposure temperature of approximately 600 ◦C was observed. Furthermore, the phosphorescence lifetime data indicated a bi-exponential signal decay with both a faster few µs decay time in the range of 3–10 µs and a slower one in the range of 30–60 µs. The recording of fluorescence and phosphorescence allowed deriving the relative signal proportion as an unbiased measure of the temperature dependence. The measurements on thermally altered bones are of particular interest for application to forensic and archeological investigations.
DOI of the first publication: 10.3390/app12031066
Link to this record: urn:nbn:de:bsz:291--ds-354699
ISSN: 2076-3417
Date of registration: 17-Feb-2022
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Systems Engineering
Professorship: NT - Prof. Dr. Karsten König
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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