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Titel: Vascularization of Microvascular Fragment Isolates from Visceral and Subcutaneous Adipose Tissue of Mice
VerfasserIn: Später, Thomas
Marschall, Julia E.
Brücker, Lea K.
Nickels, Ruth M.
Metzger, Wolfgang
Menger, Michael D.
Laschke, Matthias W.
Sprache: Englisch
Titel: Tissue Engineering and Regenerative Medicine
Bandnummer: 19
Heft: 1
Seiten: 161–175
Verlag/Plattform: Springer Nature
Erscheinungsjahr: 2022
Freie Schlagwörter: Tissue engineering
Microvascular fragments
Vascularization
Angiogenesis
Scaffold
DDC-Sachgruppe: 610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Background: Adipose tissue-derived microvascular fragments (MVF) represent effective vascularization units for tissue engineering. Most experimental studies in rodents exclusively use epididymal adipose tissue as a visceral fat source for MVF isolation. However, in future clinical practice, MVF may be rather isolated from liposuctioned subcutaneous fat tissue of patients. Therefore, we herein compared the vascularization characteristics of MVF isolates from visceral and subcutaneous fat tissue of murine origin. Methods: MVF isolates were generated from visceral and subcutaneous fat tissue of donor mice using two different enzymatic procedures. For in vivo analyses, the MVF isolates were seeded onto collagen-glycosaminoglycan scaffolds and implanted into full-thickness skin defects within dorsal skinfold chambers of recipient mice. Results: By means of the two isolation procedures, we isolated a higher number of MVF from visceral fat tissue when compared to subcutaneous fat tissue, while their length distribution, viability and cellular composition were comparable in both groups. Intravital fluorescence microscopy as well as histological and immunohistochemical analyses revealed a significantly reduced vascularization of implanted scaffolds seeded with subcutaneous MVF isolates when compared to implants seeded with visceral MVF isolates. Light and scanning electron microscopy showed that this was due to high amounts of undigested connective tissue within the subcutaneous MVF isolates, which clogged the scaffold pores and prevented the interconnection of individual MVF into new microvascular networks. Conclusion: These findings indicate the need for improved protocols to generate connective tissue-free MVF isolates from subcutaneous fat tissue for future translational studies.
DOI der Erstveröffentlichung: 10.1007/s13770-021-00391-8
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-353860
hdl:20.500.11880/32301
http://dx.doi.org/10.22028/D291-35386
ISSN: 2212-5469
1738-2696
Datum des Eintrags: 3-Feb-2022
Fakultät: M - Medizinische Fakultät
Fachrichtung: M - Chirurgie
Professur: M - Prof. Dr. Michael D. Menger
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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