Please use this identifier to cite or link to this item: doi:10.22028/D291-35386
Title: Vascularization of Microvascular Fragment Isolates from Visceral and Subcutaneous Adipose Tissue of Mice
Author(s): Später, Thomas
Marschall, Julia E.
Brücker, Lea K.
Nickels, Ruth M.
Metzger, Wolfgang
Menger, Michael D.
Laschke, Matthias W.
Language: English
Title: Tissue Engineering and Regenerative Medicine
Volume: 19
Issue: 1
Pages: 161–175
Publisher/Platform: Springer Nature
Year of Publication: 2022
Free key words: Tissue engineering
Microvascular fragments
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Background: Adipose tissue-derived microvascular fragments (MVF) represent effective vascularization units for tissue engineering. Most experimental studies in rodents exclusively use epididymal adipose tissue as a visceral fat source for MVF isolation. However, in future clinical practice, MVF may be rather isolated from liposuctioned subcutaneous fat tissue of patients. Therefore, we herein compared the vascularization characteristics of MVF isolates from visceral and subcutaneous fat tissue of murine origin. Methods: MVF isolates were generated from visceral and subcutaneous fat tissue of donor mice using two different enzymatic procedures. For in vivo analyses, the MVF isolates were seeded onto collagen-glycosaminoglycan scaffolds and implanted into full-thickness skin defects within dorsal skinfold chambers of recipient mice. Results: By means of the two isolation procedures, we isolated a higher number of MVF from visceral fat tissue when compared to subcutaneous fat tissue, while their length distribution, viability and cellular composition were comparable in both groups. Intravital fluorescence microscopy as well as histological and immunohistochemical analyses revealed a significantly reduced vascularization of implanted scaffolds seeded with subcutaneous MVF isolates when compared to implants seeded with visceral MVF isolates. Light and scanning electron microscopy showed that this was due to high amounts of undigested connective tissue within the subcutaneous MVF isolates, which clogged the scaffold pores and prevented the interconnection of individual MVF into new microvascular networks. Conclusion: These findings indicate the need for improved protocols to generate connective tissue-free MVF isolates from subcutaneous fat tissue for future translational studies.
DOI of the first publication: 10.1007/s13770-021-00391-8
Link to this record: urn:nbn:de:bsz:291--ds-353860
ISSN: 2212-5469
Date of registration: 3-Feb-2022
Faculty: M - Medizinische Fakultät
Department: M - Chirurgie
Professorship: M - Prof. Dr. Michael D. Menger
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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