Please use this identifier to cite or link to this item: doi:10.22028/D291-35298
Title: Improved Vascularization and Survival of White Compared to Brown Adipose Tissue Grafts in the Dorsal Skinfold Chamber
Author(s): Weinzierl, Andrea
Harder, Yves
Schmauss, Daniel
Ampofo, Emmanuel
Menger, Michael D.
Laschke, Matthias W.
Language: English
Title: Biomedicines
Volume: 10
Issue: 1
Publisher/Platform: MDPI
Year of Publication: 2021
Free key words: brown adipose tissue
white adipose tissue
fat graft
dorsal skinfold chamber
intravital fluorescence microscopy
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Fat grafting is a frequently applied procedure in plastic surgery for volume reconstruction. Moreover, the transplantation of white adipose tissue (WAT) and brown adipose tissue (BAT) in creasingly gains interest in preclinical research for the treatment of obesity-related metabolic defects. Therefore, we herein directly compared the vascularization capacity and survival of WAT and BAT grafts. For this purpose, size-matched grafts isolated from the inguinal WAT pad and the interscapu lar BAT depot of C57BL/6N donor mice were syngeneically transplanted into the dorsal skinfold chamber of recipient animals. The vascularization and survival of the grafts were analyzed by means of intravital fluorescence microscopy, histology, and immunohistochemistry over an observation period of 14 days. WAT grafts showed an identical microvascular architecture and functional mi crovessel density as native WAT. In contrast, BAT grafts developed an erratic microvasculature with a significantly lower functional microvessel density when compared to native BAT. Accordingly, they also contained a markedly lower number of CD31-positive microvessels, which was associated with a massive loss of perilipin-positive adipocytes. These findings indicate that in contrast to WAT grafts, BAT grafts exhibit an impaired vascularization capacity and survival, which may be due to their higher metabolic demand. Hence, future studies should focus on the establishment of strategies to improve the engraftment of transplanted BAT.
DOI of the first publication: 10.3390/biomedicines10010023
Link to this record: urn:nbn:de:bsz:291--ds-352981
ISSN: 2227-9059
Date of registration: 26-Jan-2022
Faculty: M - Medizinische Fakultät
Department: M - Chirurgie
Professorship: M - Prof. Dr. Michael D. Menger
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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