Please use this identifier to cite or link to this item: doi:10.22028/D291-35022
Title: Photodeagradable hydrogels for tissue gluing
Author(s): Villiou, Maria
Language: English
Year of Publication: 2020
DDC notations: 500 Science
540 Chemistry
570 Life sciences, biology
600 Technology
610 Medicine and health
620 Engineering and machine engineering
660 Chemical engineering
Publikation type: Dissertation
Abstract: Hydrogel biomaterials for wound care dressing and tissue gluing need to adhere to tissue and on-demand disappear. Advanced tissue adhesives also envision the encapsulation of therapeutic drugs or cells to promote the healing process. The design of hydrogels with all these functionalities is challenging. In this PhD, hydrogels that fulfil several of the previous properties for wound dressing at reasonable chemical complexity is presented. These hydrogels can be formed in situ and encapsulate cells, they can adhere to tissue and detach after use by light exposure at cytocompatible doses. The developed photodegradable hydrogels are based on 4-star PEG end-catechol precursors for crosslinking, and intercalate photocleavable o-nitrobenzyl groups in their structure. These gels can form at mild oxidative conditions and encapsulate cells or microparticles. UV-vis light exposure (λ= 365 or 405 nm) photocleavables the nitrobenzyl moiety and promotes degradation. This can occur at cytocompatible doses, and enables on-demand detachment from tissue and release of the encapsulated materials or cells. These biomaterials are interesting for the development of advanced tissue adhesives and cell therapies, by expanding the range of functionality of existing choices.
Die entwickelten photodegradierbaren Hydrogele basieren auf 4-Stern-PEG, das in dieser Arbeit chemisch mit endständigen Catecholgruppen und photospaltbaren o-Nitrobenzylgruppen modifiziert wurde. Die Vernetzung erfolgt über die Catecholgruppen unter oxidativen Bedingungen in HEPES-Puffer mit 9-18 mM Natriumperiodat als Oxidationsmittel. Diese Bedingungen sind mild genug, um lebende Zellen oder Mikropartikeln in das Material einzubetten. Bei Belichtung mit UV (λ 365 nm) oder sichtbarem Licht (λ 405 nm) in zytokompatiblen Lichtdosen fördert die photospaltbare Nitrobenzyleinheit den Hydrogelabbau, was die on-demand Freisetzung von Zellen und das Ablösen vom Gewebe ermöglicht. Diese Biomaterialien sind interessant für die Entwicklung fortschrittlicher Gewebeklebstoffe und Zelltherapien, und erweitern den Funktionsumfang gegenüber bisherigen Auswahlmöglichkeiten.
Link to this record: urn:nbn:de:bsz:291--ds-350220
Advisor: del Campo, Aranzazu
Date of oral examination: 8-Sep-2021
Date of registration: 30-Nov-2021
Third-party funds sponsorship: The research presented in this doctoral thesis has been performed at the INM-Leibniz- Institute for New Materials (Saarbrücken) and was funded by the European Union within the Marie Sklodowska-Curie Innovative Training School (BioSmartTrainee, Project No. 642861).
Sponsorship ID: European Union within the Marie Sklodowska-Curie Innovative Training School (BioSmartTrainee, Project No. 642861)
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Materialwissenschaft und Werkstofftechnik
Professorship: NT - Prof. Dr. Aránzazu del Campo
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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