Please use this identifier to cite or link to this item: doi:10.22028/D291-35027
Title: Human skin aging is associated with increased expression of the histone variant H2A.J in the epidermis
Author(s): Rübe, Claudia E.
Bäumert, Caroline
Schuler, Nadine
Isermann, Anna
Schmal, Zoé
Glanemann, Matthias
Mann, Carl
Scherthan, Harry
Language: English
Title: npj Aging and Mechanisms of Disease
Volume: 7
Issue: 1
Publisher/Platform: Springer Nature
Year of Publication: 2021
Free key words: Ageing
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Cellular senescence is an irreversible growth arrest that occurs as a result of damaging stimuli, including DNA damage and/or telomere shortening. Here, we investigate histone variant H2A.J as a new biomarker to detect senescent cells during human skin aging. Skin biopsies from healthy volunteers of different ages (18–90 years) were analyzed for H2A.J expression and other parameters involved in triggering and/or maintaining cellular senescence. In the epidermis, the proportions of H2A.J-expressing keratinocytes increased from ≈20% in young to ≈60% in aged skin. Inverse correlations between Ki67- and H2A.J staining in germinative layers may reflect that H2A.J-expressing cells having lost their capacity to divide. As cellular senescence is triggered by DNA-damage signals, persistent 53BP1-foci, telomere lengths, and telomere-associated damage foci were analyzed in epidermal keratinocytes. Only slight age-related telomere attrition and few persistent nuclear 53BP1-foci, occasionally colocalizing with telomeres, suggest that unprotected telomeres are not a significant cause of senescence during skin aging. Quantification of integrin-α6+ basal cells suggests that the number and function of stem/progenitor cells decreased during aging and their altered proliferation capacities resulted in diminished tissue renewal with epidermal thinning. Collectively, our findings suggest that H2A.J is a sensitive marker of epidermal aging in human skin.
DOI of the first publication: 10.1038/s41514-021-00060-z
Link to this record: urn:nbn:de:bsz:291--ds-350270
ISSN: 2056-3973
Date of registration: 23-Nov-2021
Description of the related object: Supplementary information
Related object:
Faculty: M - Medizinische Fakultät
Department: M - Chirurgie
M - Radiologie
Professorship: M - Prof. Dr. Matthias Glanemann
M - Prof. Dr. Christian Rübe
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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