Bitte benutzen Sie diese Referenz, um auf diese Ressource zu verweisen: doi:10.22028/D291-34834
Titel: Early Changes in Exo- and Endocytosis in the EAE Mouse Model of Multiple Sclerosis Correlate with Decreased Synaptic Ribbon Size and Reduced Ribbon-Associated Vesicle Pools in Rod Photoreceptor Synapses
VerfasserIn: Kesharwani, Ajay
Schwarz, Karin
Dembla, Ekta
Dembla, Mayur
Schmitz, Frank
Sprache: Englisch
Titel: International Journal of Molecular Sciences
Bandnummer: 22
Heft: 19
Verlag/Plattform: MDPI
Erscheinungsjahr: 2021
Freie Schlagwörter: multiple sclerosis
optic neuritis
EAE mouse model
rod photoreceptor
ribbon synapses
synaptic ribbon
exocytosis
endocytosis
dynamin
phospho-dynamin
DDC-Sachgruppe: 610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Multiple sclerosis (MS) is an inflammatory disease of the central nervous system that finally leads to demyelination. Demyelinating optic neuritis is a frequent symptom in MS. Recent studies also revealed synapse dysfunctions in MS patients and MS mouse models. We previously reported alterations of photoreceptor ribbon synapses in the experimental auto-immune encephalomyelitis (EAE) mouse model of MS. In the present study, we found that the previously observed decreased imunosignals of photoreceptor ribbons in early EAE resulted from a decrease in synaptic ribbon size, whereas the number/density of ribbons in photoreceptor synapses remained unchanged. Smaller photoreceptor ribbons are associated with fewer docked and ribbon-associated vesicles. At a functional level, depolarization-evoked exocytosis as monitored by optical recording was diminished even as early as on day 7 after EAE induction. Moreover compensatory, post-depolarization endocytosis was decreased. Decreased post-depolarization endocytosis in early EAE correlated with diminished synaptic enrichment of dynamin3. In contrast, basal endocytosis in photoreceptor synapses of resting non-depolarized retinal slices was increased in early EAE. Increased basal endocytosis correlated with increased de-phosphorylation of dynamin1. Thus, multiple endocytic pathways in photoreceptor synapse are differentially affected in early EAE and likely contribute to the observed synapse pathology in early EAE.
DOI der Erstveröffentlichung: 10.3390/ijms221910789
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-348347
hdl:20.500.11880/31849
http://dx.doi.org/10.22028/D291-34834
ISSN: 1422-0067
Datum des Eintrags: 13-Okt-2021
Fakultät: M - Medizinische Fakultät
Fachrichtung: M - Anatomie und Zellbiologie
Professur: M - Prof. Dr. Frank Schmitz
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

Dateien zu diesem Datensatz:
Datei Beschreibung GrößeFormat 
ijms-22-10789-v2.pdf17,88 MBAdobe PDFÖffnen/Anzeigen


Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons Creative Commons