Please use this identifier to cite or link to this item: doi:10.22028/D291-34599
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Title: Oscillatory Microrheology, Creep Compliance and Stress Relaxation of Biological Cells Reveal Strong Correlations as Probed by Atomic Force Microscopy
Author(s): Flormann, Daniel A. D.
Anton, C.
Pohland, M. O.
Bautz, Y.
Kaub, Kevin
Terriac, Emmanuel
Schäffer, T. E.
Rheinlaender, Johannes
Janshoff, A.
Ott, Albrecht
Lautenschläger, Franziska
Language: English
Title: Frontiers in physics
Volume: 9
Issue: 472
Publisher/Platform: Frontiers
Year of Publication: 2021
Publikation type: Journal Article
Abstract: The mechanical properties of cells are important for many biological processes, including wound healing, cancers, and embryogenesis. Currently, our understanding of cell mechanical properties remains incomplete. Different techniques have been used to probe different aspects of the mechanical properties of cells, among them microplate rheology, optical tweezers, micropipette aspiration, and magnetic twisting cytometry. These techniques have given rise to different theoretical descriptions, reaching from simple Kelvin-Voigt or Maxwell models to fractional such as power law models, and their combinations. Atomic force microscopy (AFM) is a flexible technique that enables global and local probing of adherent cells. Here, using an AFM, we indented single retinal pigmented epithelium cells adhering to the bottom of a culture dish. The indentation was performed at two locations: above the nucleus, and towards the periphery of the cell. We applied creep compliance, stress relaxation, and oscillatory rheological tests to wild type and drug modified cells. Considering known fractional and semi-fractional descriptions, we found the extracted parameters to correlate. Moreover, the Young’s modulus as obtained from the initial indentation strongly correlated with all of the parameters from the applied power-law descriptions. Our study shows that the results from different rheological tests are directly comparable. This can be used in the future, for example, to reduce the number of measurements in planned experiments. Apparently, under these experimental conditions, the cells possess a limited number of degrees of freedom as their rheological properties change.
DOI of the first publication: 10.3389/fphy.2021.711860
URL of the first publication:
Link to this record: hdl:20.500.11880/31815
ISSN: 2296-424X
Date of registration: 4-Oct-2021
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Physik
Professorship: NT - Jun.-Prof. Dr. Franziska Lautenschläger
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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