Please use this identifier to cite or link to this item: doi:10.22028/D291-34738
Title: Darbepoetin-α increases the blood volume flow in transplanted pancreatic islets in mice
Author(s): Menger, Maximilian M.
Nalbach, Lisa
Wrublewsky, Selina
Glanemann, Matthias
Gu, Yuan
Laschke, Matthias W.
Menger, Michael D.
Ampofo, Emmanuel
Language: English
Title: Acta Diabetologica
Volume: 57
Issue: 8
Pages: 1009–1018
Publisher/Platform: Springer Nature
Year of Publication: 2020
Free key words: Darbepoetin-α
Islets
Transplantation
Revascularization
Diabetes
Angiogenesis
Endothelial cells
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Aims The minimal-invasive transplantation of pancreatic islets is a promising approach to treat diabetes mellitus type 1. However, islet transplantation is still hampered by the insufficient process of graft revascularization, leading to a poor clinical outcome. Accordingly, the identification of novel compounds, which accelerate and improve the revascularization of transplanted islets, is of great clinical interest. Previous studies have shown that darbepoetin (DPO)-α, a long lasting analogue of erythropoietin, is capable of promoting angiogenesis. Hence, we investigated in this study whether DPO improves the revascularization of transplanted islets. Methods Islets were isolated from green fluorescent protein-positive FVB/N donor mice and transplanted into dorsal skinfold chambers of FVB/N wild-type animals, which were treated with DPO low dose (2.5 µg/kg), DPO high dose (10 µg/kg) or vehicle (control). The revascularization was assessed by repetitive intravital fluorescence microscopy over an observation period of 14 days. Subsequently, the cellular composition of the grafts was analyzed by immunohistochemistry. Results The present study shows that neither low- nor high-dose DPO treatment accelerates the revascularization of free pancreatic islet grafts. However, high-dose DPO treatment increased the blood volume flow of the transplanted islet. Conclusions These findings demonstrated that DPO treatment does not affect the revascularization of transplanted islets. However, the glycoprotein increases the blood volume flow of the grafts, which results in an improved microvascular function and may facilitate successful transplantation.
DOI of the first publication: 10.1007/s00592-020-01512-w
Link to this record: urn:nbn:de:bsz:291--ds-347386
hdl:20.500.11880/31788
http://dx.doi.org/10.22028/D291-34738
ISSN: 1432-5233
0940-5429
Date of registration: 24-Sep-2021
Faculty: M - Medizinische Fakultät
Department: M - Chirurgie
Professorship: M - Prof. Dr. Matthias Glanemann
M - Prof. Dr. Michael D. Menger
M - Prof. Dr. Tim Pohlemann
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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