Please use this identifier to cite or link to this item: doi:10.22028/D291-34730
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Title: T cell stiffness is enhanced upon formation of immunological synapse
Author(s): Jung, Philipp
Zhou, Xiangda
Iden, Sandra
Bischoff, Markus
Qu, Bin
Language: English
Title: eLife
Volume: 10
Publisher/Platform: eLife Sciences Publications
Year of Publication: 2021
Publikation type: Journal Article
Abstract: T cells are activated by target cells via an intimate contact, termed immunological synapse (IS). Cellular mechanical properties, especially stiffness, are essential to regulate cell functions. However, T cell stiffness at a subcellular level at the IS still remains largely elusive. In this work, we established an atomic force microscopy (AFM)-based elasticity mapping method on whole T cells to obtain an overview of the stiffness with a resolution of ~60 nm. Using primary human CD4+ T cells, we show that when T cells form IS with stimulating antibody-coated surfaces, the lamellipodia are stiffer than the cell body. Upon IS formation, T cell stiffness is enhanced both at the lamellipodia and on the cell body. Chelation of intracellular Ca2+ abolishes IS-induced stiffening at the lamellipodia but has no influence on cell-body-stiffening, suggesting different regulatory mechanisms of IS-induced stiffening at the lamellipodia and the cell body.
DOI of the first publication: 10.7554/eLife.66643
URL of the first publication:
Link to this record: hdl:20.500.11880/31785
ISSN: 2050-084X
Date of registration: 23-Sep-2021
Faculty: M - Medizinische Fakultät
NT - Naturwissenschaftlich- Technische Fakultät
Department: M - Biophysik
Professorship: NT - Keiner Professur zugeordnet
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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