Please use this identifier to cite or link to this item: doi:10.22028/D291-34517
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Title: Scanning electron microscopy preparation of the cellular actin cortex: A quantitative comparison between critical point drying and hexamethyldisilazane drying
Author(s): Schu, Moritz
Terriac, Emmanuel
Koch, Marcus
Paschke, Stephan
Lautenschläger, Franziska
Flormann, Daniel A. D.
Language: English
Volume: 16
Issue: 7
Publisher/Platform: PLOS
Year of Publication: 2021
Publikation type: Journal Article
Abstract: The cellular cortex is an approximately 200-nm-thick actin network that lies just beneath the cell membrane. It is responsible for the mechanical properties of cells, and as such, it is involved in many cellular processes, including cell migration and cellular interactions with the environment. To develop a clear view of this dense structure, high-resolution imaging is essential. As one such technique, electron microscopy, involves complex sample preparation procedures. The final drying of these samples has significant influence on potential artifacts, like cell shrinkage and the formation of artifactual holes in the actin cortex. In this study, we compared the three most used final sample drying procedures: critical-point drying (CPD), CPD with lens tissue (CPD-LT), and hexamethyldisilazane drying. We show that both hexamethyldisilazane and CPD-LT lead to fewer artifactual mesh holes within the actin cortex than CPD. Moreover, CPD-LT leads to significant reduction in cell height compared to hexamethyldisilazane and CPD. We conclude that the final drying procedure should be chosen according to the reduction in cell height, and so CPD-LT, or according to the spatial separation of the single layers of the actin cortex, and so hexamethyldisilazane.
DOI of the first publication: 10.1371/journal.pone.0254165
URL of the first publication:
Link to this record: hdl:20.500.11880/31678
ISSN: 1932-6203
Date of registration: 1-Sep-2021
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Physik
Professorship: NT - Jun.-Prof. Dr. Franziska Lautenschläger
Collections:Die Universitätsbibliographie

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