Please use this identifier to cite or link to this item: doi:10.22028/D291-34590
Title: Cyclofaulknamycin with the Rare Amino Acid D-capreomycidine Isolated from a Well-Characterized Streptomyces albus Strain
Author(s): Horbal, Liliya
Stierhof, Marc
Palusczak, Anja
Zapp, Josef
Luzhetskyy, Andriy
Eckert, Nikolas
Language: English
Title: Microorganisms
Volume: 9
Issue: 8
Publisher/Platform: MDPI
Year of Publication: 2021
Free key words: D-capreomycidine
DDC notations: 500 Science
Publikation type: Journal Article
Abstract: Targeted genome mining is an efficient method of biosynthetic gene cluster prioritization within constantly growing genome databases. Using two capreomycidine biosynthesis genes, alphaketoglutarate-dependent arginine beta-hydroxylase and pyridoxal-phosphate-dependent aminotransferase, we identified two types of clusters: one type containing both genes involved in the biosynthesis of the abovementioned moiety, and other clusters including only arginine hydroxylase. Detailed analysis of one of the clusters, the flk cluster from Streptomyces albus, led to the identification of a cyclic peptide that contains a rare D-capreomycidine moiety for the first time. The absence of the pyridoxal-phosphate-dependent aminotransferase gene in the flk cluster is compensated by the XNR_1347 gene in the S. albus genome, whose product is responsible for biosynthesis of the abovementioned nonproteinogenic amino acid. Herein, we report the structure of cyclofaulknamycin and the characteristics of its biosynthetic gene cluster, biosynthesis and bioactivity profile.
DOI of the first publication: 10.3390/microorganisms9081609
Link to this record: urn:nbn:de:bsz:291--ds-345909
ISSN: 2076-2607
Date of registration: 30-Aug-2021
Description of the related object: Supplementary Materials
Related object:
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Pharmazie
Professorship: NT - Prof. Dr. Alexandra K. Kiemer
NT - Prof. Dr. Andriy Luzhetskyy
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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