Please use this identifier to cite or link to this item: doi:10.22028/D291-34187
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Title: Nanomechanics of self-assembled DNA building blocks
Author(s): Penth, Michael
Schellnhuber, Kordula
Bennewitz, Roland
Blass, Johanna
Language: English
Title: Nanoscale
Volume: 13
Issue: 20
Startpage: 9371
Endpage: 9380
Publisher/Platform: RSC
Year of Publication: 2021
Publikation type: Journal Article
Abstract: DNA has become a powerful platform to design functional nanodevices. DNA nanodevices are often composed of self-assembled DNA building blocks that differ significantly from the structure of native DNA. In this study, we present Flow Force Microscopy as a massively parallel approach to study the nanomechanics of DNA self-assemblies on the single-molecular level. The high-throughput experiments performed in a simple microfluidic channel enable statistically meaningful studies with nanometer scale precision in a time frame of several minutes. A surprisingly high flexibility was observed for a typical construct used in DNA origami, reflected in a persistence length of 10.2 nm, a factor of five smaller than for native DNA. The enhanced flexibility is attributed to the discontinuous backbone of DNA self-assemblies that facilitate base pair opening by thermal fluctuations at the end of hybridized oligomers. We believe that the results will contribute to the fundamental understanding of DNA nanomechanics and help to improve the design of DNA nanodevices with applications in biological analysis and clinical research.
DOI of the first publication: 10.1039/D0NR06865A
URL of the first publication: https://pubs.rsc.org/en/content/articlelanding/2021/nr/d0nr06865a#!divAbstract
Link to this record: hdl:20.500.11880/31416
http://dx.doi.org/10.22028/D291-34187
ISSN: 2040-3372
2040-3364
Date of registration: 23-Jun-2021
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Physik
Professorship: NT - Keiner Professur zugeordnet
Collections:Die Universitätsbibliographie

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