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Titel: Integrated microRNA and mRNA Expression Profiling Identifies Novel Targets and Networks Associated with Ebstein’s Anomaly
VerfasserIn: Abu-Halima, Masood
Wagner, Viktoria
Becker, Lea Simone
Ayesh, Basim M.
Abd El-Rahman, Mohammed
Fischer, Ulrike
Meese, Eckart
Abdul-Khaliq, Hashim
Sprache: Englisch
Titel: Cells
Bandnummer: 10
Heft: 5
Verlag/Plattform: MDPI
Erscheinungsjahr: 2021
Freie Schlagwörter: microRNA
mRNA
integration analysis
congenital heart defect
Ebstein’s anomaly
DDC-Sachgruppe: 610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Little is known about abundance level changes of circulating microRNAs (miRNAs) and messenger RNAs (mRNA) in patients with Ebstein’s anomaly (EA). Here, we performed an integrated analysis to identify the differentially abundant miRNAs and mRNA targets and to identify the potential therapeutic targets that might be involved in the mechanisms underlying EA. A large panel of human miRNA and mRNA microarrays were conducted to determine the genome-wide expression profiles in the blood of 16 EA patients and 16 age and gender-matched healthy control volunteers (HVs). Differential abundance level of single miRNA and mRNA was validated by RealTime quantitative PCR (RT-qPCR). Enrichment analyses of altered miRNA and mRNA abundance levels were identified using bioinformatics tools. Altered miRNA and mRNA abundance levels were observed between EA patients and HVs. Among the deregulated miRNAs and mRNAs, 76 miRNAs (49 lower abundance and 27 higher abundance, fold-change of ≥2) and 29 mRNAs (25 higher abundance and 4 lower abundance, fold-change of ≥1.5) were identified in EA patients compared to HVs. Bioinformatics analysis identified 37 pairs of putative miRNA-mRNA interactions. The majority of the correlations were detected between the lower abundance level of miRNA and higher abundance level of mRNA, except for let-7b-5p, which showed a higher abundance level and their target gene, SCRN3, showed a lower abundance level. Pathway enrichment analysis of the deregulated mRNAs identified 35 significant pathways that are mostly involved in signal transduction and cellular interaction pathways. Our findings provide new insights into a potential molecular biomarker(s) for the EA that may guide the development of novel targeting therapies.
DOI der Erstveröffentlichung: 10.3390/cells10051066
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-341042
hdl:20.500.11880/31361
http://dx.doi.org/10.22028/D291-34104
ISSN: 2073-4409
Datum des Eintrags: 26-Mai-2021
Bezeichnung des in Beziehung stehenden Objekts: Supplementary Materials
In Beziehung stehendes Objekt: https://www.mdpi.com/article/10.3390/cells10051066/s1
Fakultät: M - Medizinische Fakultät
Fachrichtung: M - Humangenetik
M - Pädiatrie
Professur: M - Prof. Dr. Hashim Abdul-Khaliq
M - Prof. Dr. Eckhart Meese
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons Creative Commons