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Titel: Impact of MiRNA-181a2 on the Clinical Course of IDH1 Wild Type Glioblastoma
VerfasserIn: Sippl, Christoph
Schoeneberger, Louisa
Teping, Fritz
Schulz-Schaeffer, Walter
Urbschat, Steffi UdsID
Ketter, Ralf
Oertel, Joachim
Sprache: Englisch
In:
Titel: Processes
Bandnummer: 9
Heft: 5
Verlag/Plattform: MDPI
Erscheinungsjahr: 2021
Freie Schlagwörter: microRNA
glioblastoma
prognosis
epigenetic
miR-181a2
DDC-Sachgruppe: 610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Background: Recently, miRNA-181a2 could be identified as a major regulator of IDH1 expression in fat tissue. The IDH1 gene, its mutation and expression have a major impact on overall survival in patients with glioblastoma. The presented study aimed to investigate the effect of miRNA181a2 on IDH1 expression in glioblastoma and on the prognosis of patients suffering from, for example, a tumor. Methods: A total of 74 glioblastoma specimens were analyzed for the expression of miRNA-181a2, acquired as fold change, using qRT-PCR. IDH1 protein expression was estimated via mRNA quantification. Eight post mortal, non-glioma related brain tissue specimens served as the control group. The results were correlated with relevant demographic and clinical aspects of the cohort. A TCGA dataset was used as an independent reference. Results: MiRNA-181a2 was significantly downregulated in tumor samples compared to the control group (p < 0.001). In the glioblastoma cohort, 63/74 (85.1%) showed an IDH1 wild type, while 11/74 (14.9%) patients harbored an IDH 1 mutation. In patients with IDH1 wild type glioblastoma, low miRNA-181a2 expression correlated with a prolonged overall survival (p = 0.019), also verifiable in an independent TCGA dataset. This correlation could not be identified for patients with an IDH1 mutation. MiRNA-181a2 expression tended to correlate inversely with IDH1 protein expression (p = 0.06). Gross total resection of the tumor was an independent marker for a prolonged survival (p = 0.03). Conclusion: MiRNA181a2 seems to be a promising prognostic marker of selective glioblastoma patients with IDH1 wild type characteristics. This effect may be mediated via direct regulation of IDH1 expression.
DOI der Erstveröffentlichung: 10.3390/pr9050728
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-341018
hdl:20.500.11880/31358
http://dx.doi.org/10.22028/D291-34101
ISSN: 2227-9717
Datum des Eintrags: 25-Mai-2021
Fakultät: M - Medizinische Fakultät
Fachrichtung: M - Neurochirurgie
M - Neuropathologie
Professur: M - Prof. Dr. Joachim Oertel
M - Prof. Dr. Walter Schulz-Schaeffer
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes



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