Please use this identifier to cite or link to this item: doi:10.22028/D291-34092
Title: Dysregulated levels of glycogen synthase kinase-3β (GSK-3β) and miR-135 in peripheral blood samples of cases with nephrotic syndrome
Author(s): Ardalan, Mohammadreza
Hejazian, Seyyedeh Mina
Sharabiyani, Hassan Fazlazar
Farnood, Farahnoosh
Ghafari Aghdam, Amirhossein
Bastami, Milad
Ahmadian, Elham
Zununi Vahed, Sepideh
Cucchiarini, Magali
Language: English
Title: PeerJ
Volume: 8
Publisher/Platform: PeerJ
Year of Publication: 2020
Free key words: Nephrotic syndrome
Focal Segmental Glomerulosclerosis
Membranous glomerulonephritis
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Background. Glycogen synthase kinase-3 (GSK-3β) is a serine/threonine kinase with multifunctions in various physiological procedures. Aberrant level of GSK-3β in kidney cells has a harmful role in podocyte injury. Methods. In this article, the expression levels of GSK-3β and one of its upstream regulators, miR-135a-5p, were measured in peripheral blood mononuclear cells (PBMCs) of cases with the most common types of nephrotic syndrome (NS); focal segmental glomerulosclerosis (FSGS) and membranous glomerulonephritis (MGN). In so doing, fifty-two cases along with twenty-four healthy controls were included based on the strict criteria. Results. Levels of GSK-3β mRNA and miR-135 were measured with quantitative realtime PCR. There were statistically significant increases in GSK-3β expression level in NS (P = 0.001), MGN (P = 0.002), and FSGS (P = 0.015) groups compared to the control group. Dysregulated levels of miR-135a-5p in PBMCs was not significant between the studied groups. Moreover, a significant decrease was observed in the expression level of miR-135a-5p in the plasma of patients with NS (P = 0.020), MGN (P = 0.040), and FSGS (P = 0.046) compared to the control group. ROC curve analysis approved a diagnostic power of GSK-3β in discriminating patients from healthy controls (AUC: 0.72, P = 0.002) with high sensitivity and specificity. Conclusions. Dysregulated levels of GSK-3β and its regulator miR-135a may participate in the pathogenesis of NS with different etiology. Therefore, more research is needed for understanding the relationship between them.
DOI of the first publication: 10.7717/peerj.10377
Link to this record: urn:nbn:de:bsz:291--ds-340928
ISSN: 2167-8359
Date of registration: 21-May-2021
Description of the related object: Supplemental Information
Related object:
Faculty: M - Medizinische Fakultät
Department: M - Orthopädie
Professorship: M - Prof. Dr. Henning Madry
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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