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Titel: Impact of Mitochondrial Genetic Variants in ND1, ND2, ND5, and ND6 Genes on Sperm Motility and Intracytoplasmic Sperm Injection (ICSI) Outcomes
VerfasserIn: Al Smadi, Mohammad A.
Hammadeh, Mohamad Eid
Solomayer, Erich
Batiha, Osamah
Altalib, Mohammad M.
Jahmani, Mohammad Y.
Shboul, Mohammad A.
Nusair, Bassam
Amor, Houda
Sprache: Englisch
Titel: Reproductive Sciences
Verlag/Plattform: Springer Nature
Erscheinungsjahr: 2021
Freie Schlagwörter: mtDNA
Asthenozoospermia
Sperm motility
Fertilization rate
Embryo cleavage score
Embryo quality score
DDC-Sachgruppe: 610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Sperm mitochondrial dysfunction causes the generation of an insufficient amount of energy needed for sperm motility. This will affect sperm fertilization capacity, and thus, most asthenozoospermic men usually require assisted reproductive techniques. The etiology of asthenozoospermia remains largely unknown. The current study aimed to investigate the effect of mitochondrial genetic variants on sperm motility and intracytoplasmic sperm injection (ICSI) outcomes. A total of 150 couples from the ICSI cycle were enrolled in this study. One hundred five of the male partners were asthenozoospermic patients, and they were subdivided into three groups according to their percentage of sperm motility, while forty-five of the male partners were normozoospermic. Genetic variants were screened using direct Sanger’s sequencing in four mitochondrial genes (nicotinamide adenine dinucleotide hydrogen (NADH) dehydrogenase 1 (ND1), NADH dehydrogenase 2 (ND2), NADH dehydrogenase 5 (ND5), and NADH dehydrogenase 6 (ND6)). We identified three significant variants: 13708G>A (rs28359178) in ND5, 4216T>C (rs1599988) in ND1, and a novel 12506T>A in ND5 with P values 0.006, 0.036, and 0.013, respectively. The medians of sperm motility, fertilization rate, embryo cleavage score, and embryo quality score were significantly different between men showing 4216T>C, 12506T>A, 13708G>A and wild type, Mann-Whitney P values for the differences in the medians were < 0.05 in all of them. The results from this study suggest that 13708G>A, 12506T>A, and 4216 T>C variants in sperm mitochondrial DNA negatively affect sperm motility and ICSI outcomes.
DOI der Erstveröffentlichung: 10.1007/s43032-020-00449-3
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-338804
hdl:20.500.11880/31195
http://dx.doi.org/10.22028/D291-33880
ISSN: 1933-7205
1933-7191
Datum des Eintrags: 20-Apr-2021
Fakultät: M - Medizinische Fakultät
Fachrichtung: M - Frauenheilkunde
Professur: M - Prof. Dr. E.-F. Solomayer
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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