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Titel: The metastatic potential of seminomatous germ cell tumours is associated with a specific microRNA pattern
VerfasserIn: Ernst, Simone
Heinzelmann, Joana
Bohle, Rainer M.
Weber, Georg
Stöckle, Michael
Junker, Kerstin
Heinzelbecker, Julia
Sprache: Englisch
Titel: Andrology
Bandnummer: 8
Heft: 6
Seiten: 1687–1698
Verlag/Plattform: Wiley
Erscheinungsjahr: 2020
Freie Schlagwörter: miRNA
prognostic biomarkers
seminoma
testicular cancer
DDC-Sachgruppe: 610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Background Seminomatous germ cell tumours (SGCT) are the most frequent malignancy in young men. Reliable prognostic biomarkers for the prediction of metastasis at diagnosis and the risk of relapse in clinical stage I (CSI) are lacking. Adjuvant therapies carry a risk of overtreatment, whereas salvage therapies have a risk of high toxicities. Thus, the identification of reliable prognostic biomarkers is highly desirable to identify patients who will benefit from early adjuvant treatment. MicroRNAs (miRNAs) regulate tumour development and progression, and their potential as biomarkers has already been proven in a variety of malignancies. Objectives The aim of our study was to define a specific miRNA expression pattern that discriminates metastatic from non‐metastatic primary SGCT. Materials and methods Total RNA was isolated from 24 formalin‐fixed paraffin‐embedded (FFPE) primary SGCT tumours (10 non‐metastatic, five metachronously and nine synchronously metastatic) and from 10 normal testicular tissue samples. Microarray analysis was performed for global miRNA expression profiling. The results were validated by quantitative real‐time polymerase chain reaction (qRT‐PCR). Statistical analysis was performed using SPSS. Results Microarray analyses revealed a specific miRNA pattern that distinguishes metastatic from non‐metastatic SGCT. Sixty‐three miRNAs were differentially expressed in metastatic compared to non‐metastatic tumours (P < .01). Microarray results were confirmed by qRT‐PCR for three out of five selected miRNAs (miR‐29c‐5p, miR‐506‐3p and miR‐371a‐5p; P < .05). All five miRNAs (miR‐29c‐5p, miR‐506‐3p, miR‐1307‐5p, miR‐371a‐5p and miR‐371a‐3p) showed differential expression between tumour and normal tissues (P < .05). Conclusion Metastatic primary SGCTs are characterized by a specific miRNA expression pattern. Therefore, specific miRNAs could represent a new tool to predict the metastatic potential in SGCT patients.
DOI der Erstveröffentlichung: 10.1111/andr.12838
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-333116
hdl:20.500.11880/30659
http://dx.doi.org/10.22028/D291-33311
ISSN: 2047-2927
2047-2919
Datum des Eintrags: 17-Feb-2021
Bezeichnung des in Beziehung stehenden Objekts: Supporting Information
In Beziehung stehendes Objekt: https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1111%2Fandr.12838&file=andr12838-sup-0001-TableS1.docx
Fakultät: M - Medizinische Fakultät
Fachrichtung: M - Urologie und Kinderurologie
M - Pathologie
Professur: M - Prof. Dr. Michael Stöckle
M - Prof. Dr. Rainer M. Bohle
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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