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Titel: Limited life cycle and cost assessment for the bioconversion of lignin-derived aromatics into adipic acid
VerfasserIn: van Duuren, Jozef B. J. H.
de Wild, Paul J.
Starck, Sören
Bradtmöller, Christian
Selzer, Mirjam
Mehlmann, Kerstin
Schneider, Roland
Kohlstedt, Michael
Poblete-Castro, Ignacio
Stolzenberger, Jessica
Barton, Nadja
Fritz, Michel
Scholl, Stephan
Venus, Joachim
Wittmann, Christoph
Sprache: Englisch
Titel: Biotechnology and Bioengineering
Bandnummer: 117
Heft: 5
Seiten: 1381–1393
Verlag/Plattform: Wiley
Erscheinungsjahr: 2020
Freie Schlagwörter: catechol
cis
cis‐muconic acid
fast‐pyrolysis
lignin
Pseudomonas putida KT2440
DDC-Sachgruppe: 570 Biowissenschaften, Biologie
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Lignin is an abundant and heterogeneous waste byproduct of the cellulosic industry, which has the potential of being transformed into valuable biochemicals via microbial fermentation. In this study, we applied a fast-pyrolysis process using softwood lignin resulting in a two-phase bio-oil containing monomeric and oligomeric aromatics without syringol. We demonstrated that an additional hydrodeoxygenation step within the process leads to an enhanced thermochemical conversion of guaiacol into catechol and phenol. After steam bath distillation, Pseudomonas putida KT2440-BN6 achieved a percent yield of cis, cis-muconic acid of up to 95 mol% from catechol derived from the aqueous phase. We next established a downstream process for purifying cis, cis-muconic acid (39.9 g/L) produced in a 42.5 L fermenter using glucose and benzoate as carbon substrates. On the basis of the obtained values for each unit operation of the empirical processes, we next performed a limited life cycle and cost analysis of an integrated biotechnological and chemical process for producing adipic acid and then compared it with the conventional petrochemical route. The simulated scenarios estimate that by attaining a mixture of catechol, phenol, cresol, and guaiacol (1:0.34:0.18:0, mol ratio), a titer of 62.5 (g/L) cis, cis-muconic acid in the bioreactor, and a controlled cooling of pyrolysis gases to concentrate monomeric aromatics in the aqueous phase, the bio-based route results in a reduction of CO2 -eq emission by 58% and energy demand by 23% with a contribution margin for the aqueous phase of up to 88.05 euro/ton. We conclude that the bio-based production of adipic acid from softwood lignins brings environmental benefits over the petrochemical procedure and is cost-effective at an industrial scale. Further research is essential to achieve the proposed cis, cis-muconic acid yield from true lignin-derived aromatics using whole-cell biocatalysts.
DOI der Erstveröffentlichung: 10.1002/bit.27299
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-332704
hdl:20.500.11880/30620
http://dx.doi.org/10.22028/D291-33270
ISSN: 1097-0290
0006-3592
Datum des Eintrags: 11-Feb-2021
Bezeichnung des in Beziehung stehenden Objekts: Supporting Information
In Beziehung stehendes Objekt: https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fbit.27299&file=bit27299-sup-0001-Supporting_Information.docx
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Biowissenschaften
Professur: NT - Prof. Dr. Christoph Wittmann
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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