Please use this identifier to cite or link to this item: doi:10.22028/D291-33212
Title: Thermosensitive Hydrogel Based on PEO-PPO-PEO Poloxamers for a Controlled In Situ Release of Recombinant Adeno-Associated Viral Vectors for Effective Gene Therapy of Cartilage Defects
Author(s): Madry, Henning
Gao, Liang
Rey-Rico, Ana
Venkatesan, Jagadeesh K.
Müller-Brandt, Kathrin
Cai, Xiaoyu
Goebel, Lars
Schmitt, Gertrud
Speicher-Mentges, Susanne
Zurakowski, David
Menger, Michael D.
Laschke, Matthias W.
Cucchiarini, Magali
Language: English
Title: Advanced Materials
Volume: 32
Issue: 2
Pages: 1-8
Publisher/Platform: Wiley
Year of Publication: 2019
Free key words: biomaterial-guided therapy
cartilage defects
gene therapy
tissue engineering
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Advanced biomaterial-guided delivery of gene vectors is an emerging and highly attractive therapeutic solution for targeted articular cartilage repair, allowing for a controlled and minimally invasive delivery of gene vectors in a spatiotemporally precise manner, reducing intra-articular vector spread and possible loss of the therapeutic gene product. As far as it is known, the very first successful in vivo application of such a biomaterial-guided delivery of a potent gene vector in an orthotopic large animal model of cartilage damage is reported here. In detail, an injectable and thermosensitive hydrogel based on poly(ethylene oxide) (PEO)-poly(propylene oxide) (PPO)-PEO poloxamers, capable of controlled release of a therapeutic recombinant adeno-associated virus (rAAV) vector overexpressing the chondrogenic sox9 transcription factor in full-thickness chondral defects, is applied in a clinically relevant minipig model in vivo. These comprehensive analyses of the entire osteochondral unit with multiple standardized evaluation methods indicate that rAAV-FLAG-hsox9/PEO-PPO-PEO hydrogel-augmented microfracture significantly improves cartilage repair with a collagen fiber orientation more similar to the normal cartilage and protects the subchondral bone plate from early bone loss.
DOI of the first publication: 10.1002/adma.201906508
Link to this record: urn:nbn:de:bsz:291--ds-332124
ISSN: 1521-4095
Date of registration: 4-Feb-2021
Description of the related object: Supporting Information
Related object:
Faculty: M - Medizinische Fakultät
Department: M - Chirurgie
M - Orthopädie
Professorship: M - Prof. Dr. Henning Madry
M - Prof. Dr. Michael D. Menger
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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