Please use this identifier to cite or link to this item: doi:10.22028/D291-33209
Title: MicroRNA signature in spermatozoa and seminal plasma of proven fertile men and in testicular tissue of men with obstructive azoospermia
Author(s): Abu-Halima, Masood
Galata, Valentina
Backes, Christina
Keller, Andreas
Hammadeh, Mohamad
Meese, Eckart
Language: English
Title: Andrologia
Volume: 52
Issue: 2
Pages: 1-12
Publisher/Platform: Wiley
Year of Publication: 2019
Free key words: male infertility
seminal plasma
testicular tissue
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: MicroRNAs (miRNAs) have recently received a significant amount of attention due to their remarkable influence on post-transcriptional gene regulation. In this study, we aim to provide a catalogue of miRNAs present in spermatozoa, seminal plasma and testicular tissue. Expression profiles of miRNA in spermatozoa and seminal plasma of 16 proven fertile men and testicular tissue of eight men with morphologically and/or histologically confirmed obstructive azoospermia were determined by microarray and RT-qPCR in combination with bioinformatics analyses. A total of 123, 156 and 133 miRNAs were consistently detected in spermatozoa, seminal plasma and testicular tissue respectively. Sixty-four miRNAs were shared across all sample types. Based on miRNAs expression level present in each group, correlation analysis showed moderate-to-strong correlations within the spermatozoa and seminal plasma samples and a wider range of correlations within the testicular tissue samples. The target genes of known miRNAs appeared to be involved in a wide range of biological processes related to reproduction, development and differentiation of germ cells. Our results suggest that there is a certain similarity between spermatozoa and seminal plasma for the relative miRNA expression changes with respect to testicular tissue and provide an overview of the miRNAs present in each sample type.
DOI of the first publication: 10.1111/and.13503
Link to this record: urn:nbn:de:bsz:291--ds-332095
ISSN: 1439-0272
Date of registration: 4-Feb-2021
Description of the related object: Supporting Information
Related object:
Faculty: M - Medizinische Fakultät
Department: M - Frauenheilkunde
M - Humangenetik
M - Medizinische Biometrie, Epidemiologie und medizinische Informatik
Professorship: M - Prof. Dr. Eckhart Meese
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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