Please use this identifier to cite or link to this item: doi:10.22028/D291-33205
Title: An additional marker for sperm DNA quality evaluation in spermatozoa of male partners of couples undergoing assisted reproduction technique (IVF/ICSI): Protamine ratio
Author(s): Amor, Houda
Shelko, Nyaz
Hamad, Mohammed F
Zeyad, Ali
Hammadeh, Mohamad Eid
Language: English
Title: Andrologia
Volume: 51
Issue: 10
Pages: 1-14
Publisher/Platform: Wiley
Year of Publication: 2019
Free key words: DNA fragmentation
male infertility
protamine deficiency
protamine ratio
sperm DNA
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: The aim of this study was to evaluate the relationship between the protamine ratio (P1/P2), DNA fragmentation of spermatozoa and protamine deficiency. Patients were grouped into fertile (G1; n = 151) and sub‐fertile (G2; n = 121). DNA fragmentation in spermatozoa was analysed by a TUNEL assay (terminal deoxynucleotidyl transferase‐mediated deoxyuridine triphosphate nick‐end labelling), and the protamination was determined by CMA3 staining, while Western blot was used to measure protamine P1 and P2. While sperm DNA fragmentation (SDF) and protamine ratio were significantly elevated in G2 compared with G1 (12.31 ± 7.01% vs. 17.5 ± 9.5%; p = .001) and (0.91 ± 0.43 vs. 0.75 ± 0.42; p = .003); respectively, the CMA3 positive showed no difference at all between G1 and G2. In G1, the CMA3 positive correlated negatively with the P1/P2 ratio and SDF (r = −.586, r = −.297; p = .001 respectively). In contrast, the protamine ratio correlated positively with SDF (r = .356; p = .001). In G2, no correlation was observed between CMA3 positive, SDF and the P1/P2 ratio but the P1/P2 ratio showed a positive correlation with SDF (r = .479; p = .001). In conclusion, the spermatozoa DNA deterioration was closely associated with abnormal protamination but showed an association with the protamine ratio, more than with CMA3 positive. Therefore, for the evaluation of DNA damage in spermatozoa, the P1/P2 ratio might act as an additional biomarker.
DOI of the first publication: 10.1111/and.13400
Link to this record: urn:nbn:de:bsz:291--ds-332058
ISSN: 1439-0272
Date of registration: 4-Feb-2021
Faculty: M - Medizinische Fakultät
Department: M - Frauenheilkunde
Professorship: M - Keiner Professur zugeordnet
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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