Please use this identifier to cite or link to this item: doi:10.22028/D291-33122
Title: Supra-Molecular Assemblies of ORAI1 at Rest Precede Local Accumulation into Puncta after Activation
Author(s): Peckys, Diana B.
Gaa, Daniel
Alansary, Dalia
Niemeyer, Barbara A.
de Jonge, Niels
Language: English
Title: International Journal of Molecular Sciences
Volume: 22
Issue: 2
Publisher/Platform: MDPI
Year of Publication: 2021
Free key words: calcium channel
protein clusters
membrane protein
liquid phase electron microscopy
single molecule
correlative microscopy
DDC notations: 500 Science
600 Technology
610 Medicine and health
Publikation type: Journal Article
Abstract: The Ca2+ selective channel ORAI1 and endoplasmic reticulum (ER)-resident STIM proteins form the core of the channel complex mediating store operated Ca2+ entry (SOCE). Using liquid phase electron microscopy (LPEM), the distribution of ORAI1 proteins was examined at rest and after SOCEactivation at nanoscale resolution. The analysis of over seven hundred thousand ORAI1 positions revealed a number of ORAI1 channels had formed STIM-independent distinct supra-molecular clusters. Upon SOCE activation and in the presence of STIM proteins, a fraction of ORAI1 assembled in micron-sized two-dimensional structures, such as the known puncta at the ER plasma membrane contact zones, but also in divergent structures such as strands, and ring-like shapes. Our results thus question the hypothesis that stochastically migrating single ORAI1 channels are trapped at regions containing activated STIM, and we propose instead that supra-molecular ORAI1 clusters fulfill an amplifying function for creating dense ORAI1 accumulations upon SOCE-activation.
DOI of the first publication: 10.3390/ijms22020799
Link to this record: urn:nbn:de:bsz:291--ds-331225
hdl:20.500.11880/30528
http://dx.doi.org/10.22028/D291-33122
ISSN: 1422-0067
Date of registration: 2-Feb-2021
Description of the related object: Supplementary Materials
Related object: https://www.mdpi.com/1422-0067/22/2/799/s1
Faculty: M - Medizinische Fakultät
NT - Naturwissenschaftlich- Technische Fakultät
Department: M - Biophysik
NT - Physik
Professorship: NT - Keiner Professur zugeordnet
M - Keiner Professur zugeordnet
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

Files for this record:
File Description SizeFormat 
ijms-22-00799-v2.pdf4,31 MBAdobe PDFView/Open


This item is licensed under a Creative Commons License Creative Commons