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Titel: Migration of Cytotoxic T Lymphocytes in 3D Collagen Matrices
VerfasserIn: Sadjadi, Zeinab
Zhao, Renping
Hoth, Markus
Qu, Bin
Rieger, Heiko
Sprache: Englisch
Titel: Biophysical journal : BJ
Bandnummer: 119
Heft: 11
Startseite: 2141
Endseite: 2152
Verlag/Plattform: Cell Press
Erscheinungsjahr: 2020
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: CD8+ cytotoxic T lymphocytes (CTL) and natural killer cells are the main cytotoxic killer cells of the human body to eliminate pathogen-infected or tumorigenic cells (also known as target cells). To find their targets, they have to navigate and migrate through complex biological microenvironments, a key component of which is the extracellular matrix (ECM). The mechanisms underlying killer cell's navigation are not well understood. To mimic an ECM, we use a matrix formed by different collagen concentrations and analyze migration trajectories of primary human CTLs. Different migration patterns are observed and can be grouped into three motility types: slow, fast, and mixed. The dynamics are well described by a two-state persistent random walk model, which allows cells to switch between slow motion with low persistence and fast motion with high persistence. We hypothesize that the slow motility mode describes CTLs creating channels through the collagen matrix by deforming and tearing apart collagen fibers and that the fast motility mode describes CTLs moving within these channels. Experimental evidence supporting this scenario is presented by visualizing migrating T cells following each other on exactly the same track and showing cells moving quickly in channel-like cavities within the surrounding collagen matrix. Consequently, the efficiency of the stochastic search process of CTLs in the ECM should strongly be influenced by a dynamically changing channel network produced by the killer cells themselves.
DOI der Erstveröffentlichung: 10.1016/j.bpj.2020.10.020
URL der Erstveröffentlichung: https://www.sciencedirect.com/science/article/abs/pii/S0006349520308250
Link zu diesem Datensatz: hdl:20.500.11880/30398
http://dx.doi.org/10.22028/D291-33092
ISSN: 1542-0086
0006-3495
Datum des Eintrags: 19-Jan-2021
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: M - Biophysik
Professur: M - Prof. Dr. Markus Hoth
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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