Please use this identifier to cite or link to this item: doi:10.22028/D291-32946
Title: 3D Printing of a Reactive Hydrogel Bio-Ink Using a Static Mixing Tool
Author(s): Puertas-Bartolomé, María
Włodarczyk-Biegun, Małgorzata K
del Campo, Aránzazu
Vázquez-Lasa, Blanca
San Román, Julio
Language: English
Title: Polymers
Volume: 12
Issue: 9
Publisher/Platform: MDPI
Year of Publication: 2020
Free key words: 3D-bioprinting
static mixer
reactive hydrogel
hyaluronic acid
DDC notations: 500 Science
540 Chemistry
Publikation type: Journal Article
Abstract: Hydrogel-based bio-inks have recently attracted more attention for 3D printing applications in tissue engineering due to their remarkable intrinsic properties, such as a cell supporting environment. However, their usually weak mechanical properties lead to poor printability and low stability of the obtained structures. To obtain good shape fidelity, current approaches based on extrusion printing use high viscosity solutions, which can compromise cell viability. This paper presents a novel bio-printing methodology based on a dual-syringe system with a static mixing tool that allows in situ crosslinking of a two-component hydrogel-based ink in the presence of living cells. The reactive hydrogel system consists of carboxymethyl chitosan (CMCh) and partially oxidized hyaluronic acid (HAox) that undergo fast self-covalent crosslinking via Schiff base formation. This new approach allows us to use low viscosity solutions since in situ gelation provides the appropriate structural integrity to maintain the printed shape. The proposed bio-ink formulation was optimized to match crosslinking kinetics with the printing process and multi-layered 3D bio-printed scaffolds were successfully obtained. Printed scaffolds showed moderate swelling, good biocompatibility with embedded cells, and were mechanically stable after 14 days of the cell culture. We envision that this straightforward, powerful, and generalizable printing approach can be used for a wide range of materials, growth factors, or cell types, to be employed for soft tissue regeneration.
DOI of the first publication: 10.3390/polym12091986
Link to this record: urn:nbn:de:bsz:291--ds-329462
ISSN: 2073-4360
Date of registration: 21-Dec-2020
Description of the related object: Supplementary Material
Related object:
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Chemie
Professorship: NT - Prof. Dr. Aránzazu del Campo
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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