Please use this identifier to cite or link to this item: doi:10.22028/D291-30743
Title: Various Stages of Immune Synapse Formation Are Differently Dependent on the Strength of the TCR Stimulus
Author(s): Estl, Michael
Blatt, Pascal
Li, Xuemei
Becherer, Ute
Chang, Hsin-Fang
Rettig, Jens
Pattu, Varsha
Language: English
Title: International Journal of Molecular Sciences
Volume: 21
Issue: 7
Publisher/Platform: MDPI
Year of Publication: 2020
Free key words: cytotoxic T lymphocyte(s)
immune synapse
supramolecular activation center
cytotoxic granule(s)
lipid bilayers
total internal reflection fluorescence microscopy
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Cytotoxic T lymphocytes (CTL) are key players of the adaptive immune system that target tumors and infected cells. A central step to that is the formation of a cell–cell contact zone between the CTL and its target called an immune synapse (IS). Here, we investigate the influence of the initial T cell receptor (TCR) trigger of a cytolytic IS on the distinct steps leading to cytotoxic granule (CG) exocytosis. We stimulated primary CTLs from mouse using lipid bilayers with varying anti-CD3 but constant ICAM concentrations. We fluorescently labeled molecular markers of distinct IS zones such as actin, CD3, granzyme B, and Synaptobrevin2 in CTLs and imaged cytolytic IS formation by total internal reflection fluorescence microscopy (TIRFM). We found that an intermediate anti-CD3 concentration of 10 µg/mL induces the fastest adhesion of CTLs to the bilayers and results in maximal CG fusion efficiency. The latency of actin ring formation, dwell time, and maximum surface area at the IS exhibit different dependencies on the stimulatory anti-CD3 concentrations. The number and surface area of CD3 clusters at the IS seem to show a different dependency to the TCR trigger when compared to their dwell time. Finally, the mode of full CG exocytosis appears to be independent of the TCR trigger.
DOI of the first publication: 10.3390/ijms21072475
Link to this record: urn:nbn:de:bsz:291--ds-307431
ISSN: 1422-0067
Date of registration: 17-Dec-2020
Description of the related object: Supplementary Materials
Related object:
Faculty: M - Medizinische Fakultät
Department: M - Physiologie
Professorship: M - Prof. Dr. Jens Rettig
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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