Please use this identifier to cite or link to this item: doi:10.22028/D291-30741
Title: Efficacy of Allicin against Plant Pathogenic Fungi and Unveiling the Underlying Mode of Action Employing Yeast Based Chemogenetic Profiling Approach
Author(s): Sarfraz, Muhammad
Nasim, Muhammad Jawad
Jacob, Claus
Gruhlke, Martin C. H.
Language: English
Title: Applied Sciences
Volume: 10
Issue: 7
Publisher/Platform: MDPI
Year of Publication: 2020
Free key words: allicin
antifungal activities
haploinsufficiency screening
chemogenetic screening
DDC notations: 500 Science
600 Technology
610 Medicine and health
Publikation type: Journal Article
Abstract: Allicin (diallylthiosulfinate) is the principal organosulfur compound present in freshly damaged garlic tissue which exhibits a wide range of biological actions including antibacterial, antifungal, antiviral and anticancer properties. The antifungal activities of allicin were investigated against plant pathogenic fungi of agriculture importance. Furthermore, a yeast genome haploinsufficiency screening was also employed to decipher the antifungal mode of action of allicin. Wildtype and 1152 yeast mutant strains (each deprived of one specific allele of an essential gene in a diploid strain) were screened against allicin. Allicin exhibited promising antifungal properties against all the tested plant pathogens. Haploinsufficiency screening revealed three hypersensitive yeast mutants with gene deletions coding for proteins involved in DNA replication, mitochondrial translation and chromatids cohesion. These processes play a vital role in the cell cycle, growth and viability of yeast cells. Taken together, the results of the present study unravel the excellent antifungal activities and mechanisms and modes of action of allicin. These findings also indicate the potential use of allicin as an alternative “green” fungicide (fumigant) in agriculture.
DOI of the first publication: 10.3390/app10072563
Link to this record: urn:nbn:de:bsz:291--ds-307413
ISSN: 2076-3417
Date of registration: 16-Dec-2020
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Pharmazie
Professorship: NT - Prof. Dr. Claus Jacob
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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