Please use this identifier to cite or link to this item: doi:10.22028/D291-32729
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Title: Label‐Free Imaging of Cholesterol Assemblies Reveals Hidden Nanomechanics of Breast Cancer Cells
Author(s): Dumitru, Andra C.
Mohammed, Danahe
Maja, Mauriane
Yang, Jinsung
Verstraeten, Sandrine
del Campo Bécares, Aránzazu
Mingeot‐Leclercq, Marie‐Paule
Tyteca, Donatienne
Alsteens, David
Language: English
Title: Advanced science
Volume: 7
Issue: 22
Publisher/Platform: Wiley
Year of Publication: 2020
Publikation type: Journal Article
Abstract: Tumor cells present profound alterations in their composition, structural organization, and functional properties. A landmark of cancer cells is an overall altered mechanical phenotype, which so far are linked to changes in their cytoskeletal regulation and organization. Evidence exists that the plasma membrane (PM) of cancer cells also shows drastic changes in its composition and organization. However, biomechanical characterization of PM remains limited mainly due to the difficulties encountered to investigate it in a quantitative and label‐free manner. Here, the biomechanical properties of PM of a series of MCF10 cell lines, used as a model of breast cancer progression, are investigated. Notably, a strong correlation between the cell PM elasticity and oncogenesis is observed. The altered membrane composition under cancer progression, as emphasized by the PM‐associated cholesterol levels, leads to a stiffening of the PM that is uncoupled from the elastic cytoskeletal properties. Conversely, cholesterol depletion of metastatic cells leads to a softening of their PM, restoring biomechanical properties similar to benign cells. As novel therapies based on targeting membrane lipids in cancer cells represent a promising approach in the field of anticancer drug development, this method contributes to deciphering the functional link between PM lipid content and disease.
DOI of the first publication: 10.1002/advs.202002643
URL of the first publication: https://onlinelibrary.wiley.com/doi/10.1002/advs.202002643
Link to this record: hdl:20.500.11880/30101
http://dx.doi.org/10.22028/D291-32729
ISSN: 2198-3844
Date of registration: 26-Nov-2020
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Chemie
Professorship: NT - Prof. Dr. Aránzazu del Campo
Collections:Die Universitätsbibliographie

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