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Titel: Yeast Chemogenetic Screening as a Tool to Unravel  the Antifungal Mode of Action of Two Selected Selenocyanates
VerfasserIn: Sarfraz, Muhammad
Nasim, Muhammad Jawad
Jacob, Claus
Gruhlke, Martin C.H.
Sprache: Englisch
Titel: Applied Sciences
Bandnummer: 9
Heft: 18
Verlag/Plattform: MDPI
Erscheinungsjahr: 2019
Freie Schlagwörter: selenocyanates
yeast mutants
Arabidopsis mutants
chemogenetic screening
oxidative stress
glutathione metabolism
DDC-Sachgruppe: 500 Naturwissenschaften
600 Technik
610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: During recent decades, selenium-containing compounds, as with the chemically similar sulfur-containing compounds, have gained considerable interest as cytotoxic and anticancer agents. Selenocyanates represent a well-established class of organic selenium compounds. These agents exhibit a wide range of biological activities. Classically, selenocyanates may cause an increase in the intracellular levels of reactive oxygen species (ROS) and exert cytotoxic activities, thus, acting as pro-oxidants. In this study, chemogenetic profiling was carried out to decipher the resistance mechanisms as central part of the antifungal mode of action against two selected selenocyanates. If a mutant line is less resistant against a compound compared to the wildtype, the gene deleted in that strain seems to be correlated with the resistance. Yeast mutants carrying gene deletions for specific redox-related protein function were employed in the chemogenetic screening. The results of screening reveal the hypersensitivity of mutants carrying deletions for glutathione pool and metabolism. To confirm the results, Arabidopsis mutants deficient in glutathione were subjected to various concentrations of selenocyanates to observe their effects on mutants and the wildtype. A significant dose dependent inhibition in Arabidopsis mutants compared to the wildtype confirmed the findings of the chemogenetic screening. The data suggest that the two representatives of organoselenium compounds cause oxidative stress in yeast cells and glutathione participates towards the development of resistance against the chemicals.
DOI der Erstveröffentlichung: 10.3390/app9183728
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-293666
hdl:20.500.11880/30026
http://dx.doi.org/10.22028/D291-29366
ISSN: 2076-3417
Datum des Eintrags: 17-Nov-2020
Bezeichnung des in Beziehung stehenden Objekts: Supplementary Materials
In Beziehung stehendes Objekt: http://www.mdpi.com/2076-3417/9/18/3728/s1
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Pharmazie
Professur: NT - Prof. Dr. Claus Jacob
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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