Please use this identifier to cite or link to this item: doi:10.22028/D291-32620
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Title: Graphene Enclosure of Chemically Fixed Mammalian Cells for Liquid-Phase Electron Microscopy
Author(s): Blach, Patricia
Keskin, Sercan
de Jonge, Niels
Language: English
Title: Journal of Visualized Experiments : JoVE
Issue: 163
Publisher/Platform: JoVE
Year of Publication: 2020
Publikation type: Journal Article
Abstract: A protocol is described for investigating the human epidermal growth factor receptor 2 (HER2) in the intact plasma membrane of breast cancer cells using scanning transmission electron microscopy (STEM). Cells of the mammalian breast cancer cell line SKBR3 were grown on silicon microchips with silicon nitride (SiN) windows. Cells were chemically fixed, and HER2 proteins were labeled with quantum dot nanoparticles (QDs), using a two-step biotin-streptavidin binding protocol. The cells were coated with multilayer graphene to maintain a hydrated state, and to protect them from electron beam damage during STEM. To examine the stability of the samples under electron beam irradiation, a dose series experiment was performed. Graphene-coated and non-coated samples were compared. Beam induced damage, in the form of bright artifacts, appeared for some non-coated samples at increased electron dose D, while no artifacts appeared on coated samples.
DOI of the first publication: 10.3791/61458
URL of the first publication: https://www.jove.com/v/61458/graphene-enclosure-chemically-fixed-mammalian-cells-for-liquid-phase
Link to this record: hdl:20.500.11880/29988
http://dx.doi.org/10.22028/D291-32620
ISSN: 1940-087X
Date of registration: 11-Nov-2020
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Physik
Professorship: NT - Keiner Professur zugeordnet
Collections:Die Universitätsbibliographie

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