Please use this identifier to cite or link to this item: doi:10.22028/D291-32461
Title: Cancer-associated fibroblasts stimulate primary tumor growth and metastatic spread in an orthotopic prostate cancer xenograft model
Author(s): Linxweiler, Johannes
Hajili, Turkan
Körbel, Christina
Berchem, Carolina
Zeuschner, Philip
Müller, Andreas
Stöckle, Michael
Menger, Michael D.
Junker, Kerstin
Saar, Matthias
Language: English
Title: Scientific Reports
Volume: 10
Issue: 1
Publisher/Platform: SpringerNature
Year of Publication: 2020
Free key words: Cancer
Medical research
Pathogenesis
Urology
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: The unique microenvironment of the prostate plays a crucial role in the development and progression of prostate cancer (PCa). We examined the effects of cancer-associated fibroblasts (CAFs) on PCa progression using patient-derived fibroblast primary cultures in a representative orthotopic xenograft model. Primary cultures of CAFs, non-cancer-associated fibroblasts (NCAFs) and benign prostate hyperplasia-associated fibroblasts (BPHFs) were generated from patient-derived tissue specimens. These fibroblasts were coinjected together with cancer cells (LuCaP136 spheroids or LNCaP cells) in orthotopic PCa xenografts to investigate their effects on local and systemic tumor progression. Primary tumor growth as well as metastatic spread to lymph nodes and lungs were significantly stimulated by CAF coinjection in LuCaP136 xenografts. When NCAFs or BPHFs were coinjected, tumor progression was similar to injection of tumor cells alone. In LNCaP xenografts, all three fibroblast types significantly stimulated primary tumor progression compared to injection of LNCaP cells alone. CAF coinjection further increased the frequency of lymph node and lung metastases. This is the first study using an orthotopic spheroid culture xenograft model to demonstrate a stimulatory effect of patient-derived CAFs on PCa progression. The established experimental setup will provide a valuable tool to further unravel the interacting mechanisms between PCa cells and their microenvironment.
DOI of the first publication: 10.1038/s41598-020-69424-x
Link to this record: urn:nbn:de:bsz:291--ds-324616
hdl:20.500.11880/29831
http://dx.doi.org/10.22028/D291-32461
ISSN: 2045-2322
Date of registration: 6-Oct-2020
Description of the related object: Supplementary information
Related object: https://doi.org/10.1038/s41598-020-69424-x
Faculty: M - Medizinische Fakultät
Department: M - Urologie und Kinderurologie
Professorship: M - Prof. Dr. Michael Stöckle
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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