Please use this identifier to cite or link to this item: doi:10.22028/D291-32014
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Title: Possibilities and Limitations of Photoactivatable Cytochalasin D for the Spatiotemporal Regulation of Actin Dynamics
Author(s): del Campo Bécares, Aránzazu
Caswell, Patrick T.
Hetmanski, Joseph H. R.
Lautenschläger, Franziska
Terriac, Emmanuel
Zhao, Shifang
Nair, Roshna V.
Language: English
Publisher/Platform: ChemRxiv
Year of Publication: 2020
Publikation type: Other
Abstract: The study of the actin cytoskeleton and related cellular processes requires tools to specifically interfere with actin dynamics in living cell cultures, ideally with spatiotemporal control and compatible with real time imaging. A phototriggerable derivative of the actin disruptor Cytochalasin D (CytoD) is described and tested here. It includes a nitroveratryloxycarbonyl (Nvoc) photoremovable protecting group (PPG) at the hydroxyl group at C7 of CytoD. The attachment of the PPG renders Nvoc-CytoD temporarily inactive, and enables light-dosed delivery of the active drug CytoD to living cells. This article presents the full structural and physicochemical characterization, the toxicity analysis. It is complemented with biological tests to show the time scales (seconds) and spatial resolution (cellular level) achievable with a UV source in a regular microscopy setup.
DOI of the first publication: 10.26434/chemrxiv.12609545.v1
URL of the first publication:
Link to this record: hdl:20.500.11880/29596
Date of registration: 27-Aug-2020
Notes: Preprint
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Physik
Professorship: NT - Jun.-Prof. Dr. Franziska Lautenschläger
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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