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Titel: Extracellular vesicles protect glucuronidase model enzymes during freeze-drying
VerfasserIn: Frank, Julia
Richter, Maximilian
de Rossi, Chiara
Lehr, Claus-Michael
Fuhrmann, Kathrin
Fuhrmann, Gregor
Sprache: Englisch
Titel: Scientific reports
Bandnummer: 8
Heft: 1
Verlag/Plattform: SpringerNature
Erscheinungsjahr: 2018
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Extracellular vesicles (EVs) are natural nanoparticles that play important roles in intercellular communication and are increasingly studied for biosignalling, pathogenesis and therapy. Nevertheless, little is known about optimal conditions for their transfer and storage, and the potential impact on preserving EV-loaded cargoes. We present the first comprehensive stability assessment of different widely available types of EVs during various storage conditions including -80 °C, 4 °C, room temperature, and freeze-drying (lyophilisation). Lyophilisation of EVs would allow easy handling at room temperature and thus significantly enhance their expanded investigation. A model enzyme, β-glucuronidase, was loaded into different types of EVs derived from mesenchymal stem cells, endothelial cells and cancer cells. Using asymmetric flow field-flow fractionation we proved that the model enzyme is indeed stably encapsulated into EVs. When assessing enzyme activity as indicator for EV stability, and in comparison to liposomes, we show that EVs are intrinsically stable during lyophilisation, an effect further enhanced by cryoprotectants. Our findings provide new insight for exploring lyophilisation as a novel storage modality and we create an important basis for standardised and advanced EV applications in biomedical research.
DOI der Erstveröffentlichung: 10.1038/s41598-018-30786-y
URL der Erstveröffentlichung: https://www.nature.com/articles/s41598-018-30786-y
Link zu diesem Datensatz: hdl:20.500.11880/29427
http://dx.doi.org/10.22028/D291-31492
ISSN: 2045-2322
Datum des Eintrags: 14-Jul-2020
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Pharmazie
Professur: NT - Prof. Dr. Claus-Michael Lehr
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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