Please use this identifier to cite or link to this item: doi:10.22028/D291-31259
Title: Micro-RNA signatures in monozygotic twins discordant for congenital heart defects
Author(s): Abu-Halima, Masood
Weidinger, Josephin
Poryo, Martin
Henn, Dominic
Keller, Andreas
Meese, Eckart
Abdul-Khaliq, Hashim
Language: English
Title: PLOS ONE
Volume: 14
Issue: 12
Publisher/Platform: PLOS
Year of Publication: 2019
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Background: MicroRNAs (miRNAs) are small RNAs regulating gene expression post-transcriptionally. Recent studies demonstrated that miRNAs are involved in the development of congenital heart defects (CHD). In this study, we aimed at identifying the specific patterns of miRNAs in blood of monozygotic twin pairs discordant for CHD and to assess whether miRNAs might be involved in the development or reflect the consequences of CHD. Methods: miRNA microarray analysis and Real-Time Quantitative PCR (RT-qPCR) were employed to determine the miRNA abundance level from 12 monozygotic twins discordant for CHD and their non-CHD co-twins (n = 12). Enrichment analyses of altered miRNAs were performed using bioinformatics tools. Results: Compared with non-CHD co-twins, profiling analysis indicated 34 miRNAs with a significant difference in abundance level (p<0.05, fold change ≥ 1.3), of which 11 miRNAs were up-regulated and 23 miRNAs were down-regulated. Seven miRNAs were validated with RT-qPCR including miR-511-3p, miR-1306-5p, miR-421, miR-4707-3p, miR-4732-3p, miR-5189-3p, and miR-890, and the results were consistent with microarray analysis. Five miRNAs namely miR-511-3p, miR-1306-5p, miR-4732-3p, miR-5189-3p, and miR-890 were found to be significantly up-regulated in twins < 10 years old. Bioinformatics analysis showed that the 7 validated miRNAs were involved in phosphatidylinositol signaling, gap junction signaling, and adrenergic signaling in cardiomyocytes.Conclusions: Our data show deregulated miRNA abundance levels in the peripheral blood of monozygotic twins discordant for CHD, and identify new candidates for further analysis, which may contribute to understanding the development of CHD in the future. Bioinformatics analysis indicated that the target genes of these miRNAs are likely involved in signaling and communication of cardiomyocytes.
DOI of the first publication: 10.1371/journal.pone.0226164
Link to this record: urn:nbn:de:bsz:291--ds-312593
hdl:20.500.11880/29293
http://dx.doi.org/10.22028/D291-31259
ISSN: 1932-6203
Date of registration: 19-Jun-2020
Faculty: M - Medizinische Fakultät
Department: M - Pädiatrie
Professorship: M - Prof. Dr. Hashim Abdul-Khaliq
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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