Please use this identifier to cite or link to this item: doi:10.22028/D291-31037
Title: Translation of two-photon microscopy to the clinic: multimodal multiphoton CARS tomography of in vivo human skin
Author(s): König, Karsten
Breunig, Hans Georg
Goncalves Batista, Ana Maria
Schindele, Andreas
Zieger, Michael
Kaatz, Martin
Language: English
Title: Journal of Biomedical Optics
Volume: 25
Issue: 1
Publisher/Platform: SPIE
Year of Publication: 2020
Free key words: Skin
Second-harmonic generation
In vivo imaging
Two photon excitation microscopy
Femtosecond phenomena
Fluorescence lifetime imaging
CARS tomography
DDC notations: 600 Technology
Publikation type: Journal Article
Abstract: Two-photon microscopes have been successfully translated into clinical imaging tools to obtain high-resolution optical biopsies for in vivo histology. We report on clinical multiphoton coherent anti-Stokes Raman spectroscopy (CARS) tomography based on two tunable ultrashort near-infrared laser beams for label-free in vivo multimodal skin imaging. The multiphoton biopsies were obtained with the compact tomograph “MPTflex-CARS” using a photonic crystal fiber, an optomechanical articulated arm, and a four-detector-360 deg measurement head. The multiphoton tomograph has been employed to patients in a hospital with diseased skin. The clinical study involved 16 subjects, 8 patients with atopic dermatitis, 4 patients with psoriasis vulgaris, and 4 volunteers served as control. Two-photon cellular autofluorescence lifetime, second harmonic generation (SHG) of collagen, and CARS of intratissue lipids/proteins have been detected with single-photon sensitivity, submicron spatial resolution, and picosecond temporal resolution. The most important signal was the autofluorescence from nicotinamide adenine dinucleotide [NAD(P)H]. The SHG signal from collagen was mainly used to detect the epidermal–dermal junction and to calculate the ratio elastin/collagen. The CARS/Raman signal provided add-on information. Based on this view on the disease-affected skin on a subcellular level, skin areas affected by dermatitis and by psoriasis could be clearly identified. Multimodal multiphoton tomographs may become important label-free clinical high-resolution imaging tools for in vivo skin histology to realize rapid early diagnosis as well as treatment control.
DOI of the first publication: 10.1117/1.JBO.25.1.014515
Link to this record: urn:nbn:de:bsz:291--ds-310373
ISSN: 1560-2281
Date of registration: 28-May-2020
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Systems Engineering
Professorship: NT - Prof. Dr. Karsten König
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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