Please use this identifier to cite or link to this item: doi:10.22028/D291-30878
Title: Effect of physical stimuli on hair follicle deposition of clobetasol-loaded Lipid Nanocarriers
Author(s): Angelo, Tamara
El-Sayed, Nesma
Jurisic, Marijas
Koenneke, Aljoscha
Gelfuso, Guilherme M
Cunha-Filho, Marcilio
Taveira, Stephania F
Lemor, Robert
Schneider, Marc
Gratieri, Tais
Language: English
Title: Scientific Reports
Volume: 10
Publisher/Platform: Springer Nature
Year of Publication: 2020
Free key words: Drug development
Translational research
DDC notations: 500 Science
Publikation type: Journal Article
Abstract: Clobetasol propionate (CLO) is a potent glucocorticoid used to treat inflammation-based skin, scalp, and hair disorders. In such conditions, hair follicles (HF) are not only the target site but can also act as drug reservoirs when certain formulations are topically applied. Recently, we have demonstrated nanostructured lipid carriers (NLC) containing CLO presenting epidermal-targeting potential. Here, the focus was evaluating the HF uptake provided by such nanoparticles in comparison to a commercial cream and investigating the influence of different physical stimuli [i.e., infrared (IR) irradiation (with and without metallic nanoparticles-MNP), ultrasound (US) (with and without vibration) and mechanical massage] on their follicular targeting potential. Nanosystems presented sizes around 180 nm (PdI < 0.2) and negative zeta potential. The formulation did not alter skin water loss measurements and was stable for at least 30 days at 5 °C. Nanoparticles released the drug in a sustained fashion for more than 3 days and increased passively about 40 times CLO follicular uptake compared to the commercial cream. Confocal images confirmed the enhanced follicular delivery. On the one hand, NLC application followed by IR for heat generation showed no benefit in terms of HF targeting even at higher temperatures generated by metallic nanoparticle heating. On the other hand, upon US treatment, CLO retention was significantly increased in deeper skin layers. The addition of mechanical vibration to the US treatment led to higher follicular accumulation compared to passive exposure to NLC without stimuli. However, from all evaluated stimuli, manual massage presented the highest follicular targeting potential, driving more than double the amount of CLO into the HF than NLC passive application. In conclusion, NLC showed great potential for delivering CLO to HF, and a simple massage was capable of doubling follicular retention.
DOI of the first publication: 10.1038/s41598-019-56760-w
Link to this record: urn:nbn:de:bsz:291--ds-308782
ISSN: 2045-2322
Date of registration: 8-May-2020
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Pharmazie
Professorship: NT - Prof. Dr. Marc Schneider
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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