Please use this identifier to cite or link to this item: doi:10.22028/D291-30790
Title: Kinetic and spectroscopic responses of pH-sensitive nanoparticles: influence of the silica matrix
Author(s): Clasen, Anne
Wenderoth, Sarah
Tavernaro, Isabella
Fleddermann, Jana
Kraegeloh, Annette
Jung, Gregor
Language: English
Title: RSC Advances
Volume: 9
Issue: 61
Startpage: 35695
Endpage: 35705
Publisher/Platform: RSC
Year of Publication: 2019
DDC notations: 540 Chemistry
Publikation type: Journal Article
Abstract: Intracellular pH sensing with fluorescent nanoparticles is an emerging topic as pH plays several roles in physiology and pathologic processes. Here, nanoparticle-sized pH sensors (diameter far below 50 nm) for fluorescence imaging have been described. Consequently, a fluorescent derivative of pH-sensitive hydroxypyrene with pKa = 6.1 was synthesized and subsequently embedded in core and core–shell silica nanoparticles via a modified Stöber process. The detailed fluorescence spectroscopic characterization of the produced nanoparticles was carried out for retrieving information about the environment within the nanoparticle core. Several steady-state and time-resolved fluorescence spectroscopic methods hint to the screening of the probe molecule from the solvent, but it sustained interactions with hydrogen bonds similar to that of water. The incorporation of the indicator dye in the water-rich silica matrix neither changes the acidity constant nor dramatically slows down the protonation kinetics. However, cladding by another SiO2 shell leads to the partial substitution of water and decelerating the response of the probe molecule toward pH. The sensor is capable of monitoring pH changes in a physiological range by using ratiometric fluorescence excitation with λex = 405 nm and λex = 488 nm, as confirmed by the confocal fluorescence imaging of intracellular nanoparticle uptake.
DOI of the first publication: 10.1039/C9RA06047B
Link to this record: urn:nbn:de:bsz:291--ds-307909
ISSN: 2046-2069
Date of registration: 24-Apr-2020
Third-party funds sponsorship: DFG
DFG JU650/7-1 and DFG JUG650/8-1
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Chemie
Professorship: NT - Prof. Dr. Gregor Jung
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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