Please use this identifier to cite or link to this item: doi:10.22028/D291-30759
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Title: Optoregulated force application to cellular receptors using molecular motors
Author(s): Zheng, Yijun
Han, Mitchell
Zhao, Renping
Blass, Johanna
Zhang, Jingnan
Zhou, Dennis W.
Colard-Itté, Jean-Rémy
Dattler, Damien
Hoth, Markus
Garcia, Andres J.
Qu, Bin
Bennewitz, Roland
Giuseppone, Nicolas
del Campo Bécares, Aránzazu
Language: English
Publisher/Platform: bioRxiv
Year of Publication: 2020
Publikation type: Other
Abstract: Mechanotransduction events in physiological environments are difficult to investigate, in part due to the lack of experimental tools to apply forces to mechanosensitive receptors remotely. Inspired by cellular mechanisms for force application (i.e. motor proteins pulling on cytoskeletal fibers), here we present a unique molecular machine that can apply forces at cell-matrix and cell-cell junctions using light as an energy source. The key actuator is a light-driven rotatory molecular motor linked to polymer chains, which is intercalated between a membrane receptor and an engineered biointerface. The light-driven actuation of the molecular motor is converted in mechanical twisting of the polymer chains, which will in turn effectively “pulls” on engaged cell membrane receptors (integrins, cadherins…) within the illuminated area. Applied forces have the adequate magnitude and occur at time scales within the relevant ranges for mechanotransduction at cell-friendly exposure conditions, as demonstrated in forcedependent focal adhesion maturation and T cell activation experiments. Our results reveal the potential of nanomotors for the manipulation of living cells at the molecular scale and demonstrate, for the first time, a functionality which at the moment cannot be achieved by any other means.
DOI of the first publication: 10.1101/2020.03.31.015198
URL of the first publication: https://www.biorxiv.org/content/10.1101/2020.03.31.015198v1
Link to this record: hdl:20.500.11880/29051
http://dx.doi.org/10.22028/D291-30759
Date of registration: 24-Apr-2020
Notes: Preprint
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Chemie
Professorship: NT - Prof. Dr. Aránzazu del Campo
Collections:UniBib – Die Universitätsbibliographie

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