Please use this identifier to cite or link to this item: doi:10.22028/D291-30401
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Title: Thiol-Methylsulfone-Based Hydrogels for 3D Cell Encapsulation
Author(s): Paez, Julieta I.
Farrukh, Aleeza
Valbuena-Mendoza, Rocío
Włodarczyk-Biegun, Małgorzata K.
del Campo Bécares, Aránzazu
Language: English
Title: ACS applied materials & interfaces
Volume: 12
Issue: 7
Startpage: 8062
Endpage: 8072
Publisher/Platform: ACS
Year of Publication: 2020
Publikation type: Journal Article
Abstract: Thiol-maleimide and thiol-vinylsulfone cross-linked hydrogels are widely used systems in 3D culture models, in spite of presenting uncomfortable reaction kinetics for cell encapsulation: too fast (seconds for thiol-maleimide) or too slow (minutes-hours for thiol-vinylsulfone). Here, we introduce the thiol-methylsulfone reaction as alternative cross-linking chemistry for cell encapsulation, particularized for PEG-hydrogels. The thiol-methylsulfone reaction occurs at high conversion and at intermediate reaction speed (seconds-minutes) under physiological pH range. These properties allow easy mixing of hydrogel precursors and cells to render homogeneous cell-laden gels at comfortable experimental time scales. The resulting hydrogels are cytocompatible and show comparable hydrolytic stability to thiol-vinylsulfone gels. They allow direct bioconjugation of thiol-derivatized ligands and tunable degradation kinetics by cross-linking with degradable peptide sequences. 3D cell culture of two cell types, fibroblasts and human umbilical vein endothelial cells (HUVECs), is demonstrated.
DOI of the first publication: 10.1021/acsami.0c00709
URL of the first publication:
Link to this record: hdl:20.500.11880/28859
ISSN: 1944-8244
Date of registration: 11-Mar-2020
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Chemie
Professorship: NT - Prof. Dr. Aránzazu del Campo
Collections:UniBib – Die Universitätsbibliographie

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