Please use this identifier to cite or link to this item: doi:10.22028/D291-30348
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Title: Targeted delivery of functionalized PLGA nanoparticles to macrophages by complexation with the yeast Saccharomyces cerevisiae
Author(s): Kiefer, Ruth
Jurisic, Marijas
Dahlem, Charlotte
Koch, Marcus
Schmitt, Manfred J.
Kiemer, Alexandra
Schneider, Marc
Breinig, Frank
Language: English
Title: Biotechnology and bioengineering
Volume: 117
Issue: 3
Startpage: 776
Endpage: 788
Publisher/Platform: Wiley
Year of Publication: 2020
Publikation type: Journal Article
Abstract: Nanoparticles (NPs) are able to deliver a variety of substances into eukaryotic cells. However, their usage is often hampered by a lack of specificity, leading to the undesired uptake of NPs by virtually all cell types. In contrast to this, yeast is known to be specifically taken up into immune cells after entering the body. Therefore, we investigated the interaction of biodegradable surface-modified poly(lactic-co-glycolic acid) (PLGA) particles with yeast cells to overcome the unspecificity of the particulate carriers. Cells of different Saccharomyces cerevisiae strains were characterized regarding their interaction with PLGA-NPs under isotonic and hypotonic conditions. The particles were shown to efficiently interact with yeast cells leading to stable NP/yeast-complexes allowing to associate or even internalize compounds. Notably, applying those complexes to a coculture model of HeLa cells and macrophages, the macrophages were specifically targeted. This novel nano-in-micro carrier system suggests itself as a promising tool for the delivery of biologically active agents into phagocytic cells combining specificity and efficiency.
DOI of the first publication: 10.1002/bit.27226
URL of the first publication: https://onlinelibrary.wiley.com/doi/abs/10.1002/bit.27226
Link to this record: hdl:20.500.11880/28788
http://dx.doi.org/10.22028/D291-30348
ISSN: 1097-0290
0006-3592
Date of registration: 26-Feb-2020
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Biowissenschaften
NT - Pharmazie
Professorship: NT - Prof. Dr. Marc Schneider
Collections:UniBib – Die Universitätsbibliographie

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