Please use this identifier to cite or link to this item: doi:10.22028/D291-30044
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Title: Glycerylphytate as an ionic crosslinker for 3D printing of multi-layered scaffolds with improved shape fidelity and biological features
Author(s): Mora-Boza, Ana
Włodarczyk-Biegun, Małgorzata K.
del Campo Bécares, Aránzazu
Vázquez-Lasa, Blanca
Román, Julio San
Language: English
Title: Biomaterials Science
Volume: 8
Issue: 1
Startpage: 506
Endpage: 516
Publisher/Platform: RSC
Year of Publication: 2020
Publikation type: Journal Article
Abstract: The fabrication of intricate and long-term stable 3D polymeric scaffolds by a 3D printing technique is still a challenge. In the biomedical field, hydrogel materials are very frequently used because of their excellent biocompatibility and biodegradability, however the improvement of their processability and mechanical properties is still required. This paper reports the fabrication of dual crosslinked 3D scaffolds using a low concentrated (<10 wt%) ink of gelatin methacryloyl (GelMA)/chitosan and a novel crosslinking agent, glycerylphytate (G1Phy) to overcome the current limitations in the 3D printing field using hydrogels. The applied methodology consisted of a first ultraviolet light (UV) photopolymerization followed by a post-printing ionic crosslinking treatment with G1Phy. This crosslinker provides a robust framework and avoids the necessity of neutralization with strong bases. The blend ink showed shear-thinning behavior and excellent printability in the form of a straight and homogeneous filament. UV curing was undertaken simultaneously to 3D deposition, which enhanced precision and shape fidelity (resolution ≈150 μm), and prevented the collapse of the subsequent printed layers (up to 28 layers). In the second step, the novel G1Phy ionic crosslinker agent provided swelling and long term stability properties to the 3D scaffolds. The multi-layered printed scaffolds were mechanically stable under physiological conditions for at least one month. Preliminary in vitro assays using L929 fibroblasts showed very promising results in terms of adhesion, spreading, and proliferation in comparison to other phosphate-based traditional crosslinkers (i.e. TPP). We envision that the proposed combination of the blend ink and 3D printing approach can have widespread applications in the regeneration of soft tissues.
DOI of the first publication: https://doi.org/10.1039/C9BM01271K
URL of the first publication: https://pubs.rsc.org/en/Content/ArticleLanding/2019/BM/C9BM01271K#!divAbstract
Link to this record: hdl:20.500.11880/28623
http://dx.doi.org/10.22028/D291-30044
ISSN: 2047-4849
2047-4830
Date of registration: 21-Jan-2020
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Chemie
Professorship: NT - Prof. Dr. Aránzazu del Campo
Collections:UniBib – Die Universitätsbibliographie

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