Please use this identifier to cite or link to this item: doi:10.22028/D291-27501
Title: Environmental Temperature Controls Accumulation of Transacting siRNAs Involved in Heterochromatin Formation
Author(s): Pirritano, Marcello
Götz, Ulrike
Karunanithi, Sivarajan
Nordström, Karl
Schulz, Marcel H.
Simon, Martin
Language: English
Title: Genes
Volume: 9
Issue: 2
Publisher/Platform: MDPI
Year of Publication: 2018
Free key words: RNA interference
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Genes or alleles can interact by small RNAs in a homology dependent manner meaning that short interfering (siRNAs) can act in trans at the chromatin level producing stable and heritable silencing phenotypes. Because of the puzzling data on endogenous paramutations, their impact contributing to adaptive evolution in a Lamarckian manner remains unknown. An increasing number of studies characterizes the underlying siRNA accumulation pathways using transgene experiments. Also in the ciliate Paramecium tetraurelia, we induce trans silencing on the chromatin level by injection of truncated transgenes. Here, we characterize the efficiency of this mechanism at different temperatures showing that silencing of the endogenous genes is temperature dependent. Analyzing different transgene constructs at different copy numbers, we dissected whether silencing efficiency is due to varying precursor RNAs or siRNA accumulation. Our data shows that silencing efficiency correlates with more efficient accumulation of primary siRNAs at higher temperatures rather than higher expression of precursor RNAs. Due to higher primary levels, secondary siRNAs also show temperature dependency and interestingly increase their relative proportion to primary siRNAs. Our data shows that efficient trans silencing on the chromatin level in P. tetraurelia depends on environmental parameters, thus being an important epigenetic factor limiting regulatory effects of siRNAs.
DOI of the first publication: 10.3390/genes9020117
Link to this record: urn:nbn:de:bsz:291--ds-275018
ISSN: 2073-4425
Date of registration: 18-Jan-2020
Faculty: M - Medizinische Fakultät
Department: M - Experimentelle und Klinische Pharmakologie und Toxikologie
M - Humangenetik
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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