Please use this identifier to cite or link to this item: doi:10.22028/D291-29500
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Title: A Photoactivatable α5β1‐Specific Integrin Ligand
Author(s): Nair, Roshna V.
Farrukh, Aleeza
del Campo Bécares, Aránzazu
Language: English
Title: ChemBioChem : a European journal of chemical biology
Volume: 19
Issue: 12
Startpage: 1280
Endpage: 1287
Publisher/Platform: Wiley-VCH
Year of Publication: 2018
Publikation type: Journal Article
Abstract: The integrin α5β1 is overexpressed in colon, breast, ovarian, lung and brain tumours, and has been identified as key component in mechanosensing. In order to study how dynamic changes in α5β1 engagement affect cellular behaviour, photoactivatable derivatives of α5β1‐specific ligands are presented in this article. A photoremovable protecting group (PRPG) was introduced into the ligand structure at a relevant position for integrin recognition. The presence of the chromophore temporarily inhibited ligand bioactivity. Light exposure at a cell‐compatible dose efficiently cleaved the protecting group and restored functionality. The photoactive ligand had an azide end‐functional group for covalent immobilisation onto biomaterials by click chemistry. Selective cell responses (attachment, spreading, migration) to the activated ligand on the surface are achieved by controlled exposure to light, at similar levels to the native ligand. Spatial and temporal control of the cellular response is demonstrated, including the possibility of in situ activation. Photoactivatable integrin‐selective ligands in model microenvironments will allow the study of cellular behaviour in response to changes in the activation of individual integrins as consequence of dynamic variations in matrix composition.
DOI of the first publication: 10.1002/cbic.201800180
URL of the first publication:
Link to this record: hdl:20.500.11880/28563
ISSN: 1439-7633
Date of registration: 13-Jan-2020
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Chemie
Professorship: NT - Prof. Dr. Aránzazu del Campo
Collections:UniBib – Die Universitätsbibliographie

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