Please use this identifier to cite or link to this item: doi:10.22028/D291-27445
Title: Genetically Encoded Voltage Indicators in Circulation Research
Author(s): Kästner, Lars
Tian, Qinghai
Kaiser, Elisabeth
Xian, Wenying
Müller, Andreas
Oberhofer, Martin
Ruppenthal, Sandra
Sinnecker, Daniel
Tsutsui, Hidekazu
Miyawaki, Atsushi
Moretti, Alessandra
Lipp, Peter
Language: English
Title: International Journal of Molecular Sciences
Volume: 16
Issue: 9
Startpage: 21626
Endpage: 21642
Publisher/Platform: MDPI
Year of Publication: 2015
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Membrane potentials display the cellular status of non-excitable cells and mediate communication between excitable cells via action potentials. The use of genetically encoded biosensors employing fluorescent proteins allows a non-invasive biocompatible way to read out the membrane potential in cardiac myocytes and other cells of the circulation system. Although the approaches to design such biosensors date back to the time when the first fluorescent-protein based Förster Resonance Energy Transfer (FRET) sensors were constructed, it took 15 years before reliable sensors became readily available. Here, we review different developments of genetically encoded membrane potential sensors. Furthermore, it is shown how such sensors can be used in pharmacological screening applications as well as in circulation related basic biomedical research. Potentials and limitations will be discussed and perspectives of possible future developments will be provided.
DOI of the first publication: 10.3390/ijms160921626
Link to this record: urn:nbn:de:bsz:291--ds-274459
ISSN: 1422-0067
Date of registration: 6-Jan-2020
Faculty: M - Medizinische Fakultät
Department: M - Anatomie und Zellbiologie
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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