Please use this identifier to cite or link to this item: doi:10.22028/D291-30039
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Title: Strength of bacterial adhesion on nanostructured surfaces quantified by substrate morphometry
Author(s): Spengler, Christian
Nolle, Friederike
Mischo, Johannes
Faidt, Thomas
Grandthyll, Samuel
Thewes, Nicolas
Koch, Marcus
Müller, Frank
Bischoff, Markus
Klatt, Michael Andreas
Jacobs, Karin
Language: English
Title: Nanoscale
Volume: 11
Issue: 42
Startpage: 19713
Endpage: 19722
Publisher/Platform: Royal Society of Chemistry (RSC)
Year of Publication: 2019
Publikation type: Journal Article
Abstract: Microbial adhesion and the subsequent formation of resilient biofilms at surfaces are decisively influenced by substrate properties, such as the topography. To date, studies that quantitatively link surface topography and bacterial adhesion are scarce, as both are not straightforward to quantify. To fill this gap, surface morphometry combined with single-cell force spectroscopy was performed on surfaces with irregular topographies on the nano-scale. As surfaces, hydrophobized silicon wafers were used that were etched to exhibit surface structures in the same size range as the bacterial cell wall molecules. The surface structures were characterized by a detailed morphometric analysis based on Minkowski functionals revealing both qualitatively similar features and quantitatively different extensions. We find that as the size of the nanostructures increases, the adhesion forces decrease in a way that can be quantified by the area of the surface that is available for the tethering of cell wall molecules. In addition, we observe a bactericidal effect, which is more pronounced on substrates with taller structures but does not influence adhesion. Our results can be used for a targeted development of 3D-structured materials for/against bio-adhesion. Moreover, the morphometric analysis can serve as a future gold standard for characterizing a broad spectrum of material structures.
DOI of the first publication: 10.1039/C9NR04375F
URL of the first publication: https://pubs.rsc.org/en/content/articlelanding/2019/nr/c9nr04375f#!divAbstract
Link to this record: hdl:20.500.11880/28413
http://dx.doi.org/10.22028/D291-30039
ISSN: 2040-3372
2040-3364
Date of registration: 3-Dec-2019
Sponsorship ID: SFB 1027 Project B2, HU1874/3-2, LA965/6-2
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Physik
Professorship: NT - Prof. Dr. Karin Jacobs
Collections:UniBib – Die Universitätsbibliographie

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