Please use this identifier to cite or link to this item: doi:10.22028/D291-29986
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Title: Membrane Tension Orchestrates Rear Retraction in Matrix-Directed Cell Migration
Author(s): Hetmanski, Joseph H. R.
de Belly, Henry
Busnelli, Ignacio
Waring, Thomas
Nair, Roshna V.
Sokleva, Vanesa
Dobre, Oana
Cameron, Angus
Gauthier, Nils
Lamaze, Christophe
Swift, Joe
del Campo Bécares, Aránzazu
Starborg, Tobias
Zech, Tobias
Goetz, Jacky G.
Paluch, Ewa K.
Schwartz, Jean-Marc
Caswell, Patrick T.
Language: English
Title: Developmental cell
Volume: 51
Issue: 4
Startpage: 460
Endpage: 475
Publisher/Platform: Cell Press
Year of Publication: 2019
Publikation type: Journal Article
Abstract: In development, wound healing, and cancer metastasis, vertebrate cells move through 3D interstitial matrix, responding to chemical and physical guidance cues. Protrusion at the cell front has been extensively studied, but the retraction phase of the migration cycle is not well understood. Here, we show that fast-moving cells guided by matrix cues establish positive feedback control of rear retraction by sensing membrane tension. We reveal a mechanism of rear retraction in 3D matrix and durotaxis controlled by caveolae, which form in response to low membrane tension at the cell rear. Caveolae activate RhoA-ROCK1/PKN2 signaling via the RhoA guanidine nucleotide exchange factor (GEF) Ect2 to control local F-actin organization and contractility in this subcellular region and promote translocation of the cell rear. A positive feedback loop between cytoskeletal signaling and membrane tension leads to rapid retraction to complete the migration cycle in fast-moving cells, providing directional memory to drive persistent cell migration in complex matrices.
DOI of the first publication: 10.1016/j.devcel.2019.09.006
URL of the first publication: https://www.sciencedirect.com/science/article/pii/S153458071930735X
Link to this record: hdl:20.500.11880/28377
http://dx.doi.org/10.22028/D291-29986
ISSN: 1878-1551
1534-5807
Date of registration: 26-Nov-2019
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Chemie
Professorship: NT - Prof. Dr. Aránzazu del Campo
Collections:UniBib – Die Universitätsbibliographie

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