Please use this identifier to cite or link to this item: doi:10.22028/D291-29971
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Title: Dextran adsorption onto red blood cells revisited: single cell quantification by laser tweezers combined with microfluidics
Author(s): Lee, Kisung
Shirshin, Evgeny
Rovnyagina, Nataliya
Yaya, Francois
Boujja, Zakaria
Priezzhev, Alexander
Wagner, Christian
Language: English
Title: Biomedical optics express
Volume: 9
Issue: 6
Startpage: 2755
Endpage: 2764
Publisher/Platform: Optical Society of America
Year of Publication: 2018
Publikation type: Journal Article
Abstract: The aggregation of red blood cells (RBC) is of importance for hemorheology, while its mechanism remains debatable. The key question is the role of the adsorption of macromolecules on RBC membranes, which may act as "bridges" between cells. It is especially important that dextran is considered to induce "bridge"-less aggregation due to the depletion forces. We revisit the dextran-RBC interaction on the single cell level using the laser tweezers combined with microfluidic technology and fluorescence microscopy. An immediate sorption of ~104 molecules of 70 kDa dextran per cell was observed. During the incubation of RBC with dextran, a gradual tenfold increase of adsorption was found, accompanied by a moderate change in the RBC deformability. The obtained data demonstrate that dextran sorption and incubation-induced changes of the membrane properties must be considered when studying RBC aggregation in vitro.
DOI of the first publication: 10.1364/BOE.9.002755
URL of the first publication: https://www.osapublishing.org/boe/abstract.cfm?URI=boe-9-6-2755
Link to this record: hdl:20.500.11880/28359
http://dx.doi.org/10.22028/D291-29971
ISSN: 2156-7085
Date of registration: 22-Nov-2019
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Physik
Professorship: NT - Prof. Dr. Christian Wagner
Collections:UniBib – Die Universitätsbibliographie

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