Please use this identifier to cite or link to this item: doi:10.22028/D291-29463
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Title: CYP109E1 from Bacillus megaterium Acts as a 24- and 25-Hydroxylase for Cholesterol
Author(s): Putkaradze, Natalia
Litzenburger, Martin
Hutter, Michael Christopher
Bernhardt, Rita
Language: English
Title: ChemBioChem : a European journal of chemical biology
Volume: 20
Issue: 5
Startpage: 655
Endpage: 658
Publisher/Platform: Wiley-VCH
Year of Publication: 2019
Publikation type: Journal Article
Abstract: In this study, the ability of CYP109E1 from Bacillus megaterium DSM319 to metabolize cholesterol was investigated. This steroid was identified as a new substrate to be converted by CYP109E1 with adrenodoxin and adrenodoxin reductase as redox partners in vitro. The biotransformation was successfully reproduced in vivo by using Bacillus megaterium cells that overexpressed CYP109E1. To enhance the production of cholesterol derivatives, an Escherichia coli based whole-cell system that harbored CYP109E1 was established. This novel system showed a 3.3-fold higher activity than that of the B. megaterium system, yielding about 45 mg L-1 of these products. Finally, the reaction products were isolated and identified to be the highly important cholesterol derivatives 24(S)- and 25-hydroxycholesterol.
DOI of the first publication: 10.1002/cbic.201800595
URL of the first publication:
Link to this record: hdl:20.500.11880/28307
ISSN: 1439-4227
Date of registration: 15-Nov-2019
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Biowissenschaften
Professorship: NT - Prof. Dr. Volkhard Helms
Collections:Die Universitätsbibliographie

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